Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   N8A75_RS13730 Genome accession   NZ_CP104878
Coordinates   2520170..2520787 (-) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain 4ZT     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2515170..2525787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A75_RS13700 (N8A75_13700) rpsT 2515471..2515737 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  N8A75_RS13705 (N8A75_13705) holA 2515752..2516795 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  N8A75_RS13710 (N8A75_13710) - 2516835..2516948 (-) 114 WP_122060479.1 hypothetical protein -
  N8A75_RS13715 (N8A75_13715) yqzM 2517025..2517159 (+) 135 WP_003229983.1 YqzM family protein -
  N8A75_RS13720 (N8A75_13720) comEC 2517200..2519530 (-) 2331 WP_046160607.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  N8A75_RS13725 (N8A75_13725) comEB 2519534..2520103 (-) 570 WP_003229978.1 ComE operon protein 2 -
  N8A75_RS13730 (N8A75_13730) comEA 2520170..2520787 (-) 618 WP_046160608.1 competence protein ComEA Machinery gene
  N8A75_RS13735 (N8A75_13735) comER 2520871..2521692 (+) 822 WP_032726195.1 late competence protein ComER -
  N8A75_RS13740 (N8A75_13740) yqeM 2521758..2522501 (-) 744 WP_046160609.1 class I SAM-dependent DNA methyltransferase -
  N8A75_RS13745 (N8A75_13745) rsfS 2522498..2522854 (-) 357 WP_014480315.1 ribosome silencing factor -
  N8A75_RS13750 (N8A75_13750) yqeK 2522872..2523432 (-) 561 WP_129110530.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  N8A75_RS13755 (N8A75_13755) nadD 2523422..2523991 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  N8A75_RS13760 (N8A75_13760) yhbY 2524003..2524293 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  N8A75_RS13765 (N8A75_13765) aroE 2524287..2525129 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=733134 N8A75_RS13730 WP_046160608.1 2520170..2520787(-) (comEA) [Bacillus subtilis strain 4ZT]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=733134 N8A75_RS13730 WP_046160608.1 2520170..2520787(-) (comEA) [Bacillus subtilis strain 4ZT]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376