Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA2_RS22915 Genome accession   NZ_CP104865
Coordinates   4928538..4930241 (+) Length   567 a.a.
NCBI ID   WP_031668596.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4923538..4935241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA2_RS22895 (PALA2_04557) nadC 4925995..4926843 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA2_RS22905 (PALA2_04559) - 4927008..4927706 (-) 699 WP_023121720.1 hypothetical protein -
  PALA2_RS22910 - 4927770..4928309 (-) 540 WP_003116248.1 pilin -
  PALA2_RS22915 (PALA2_04560) pilB 4928538..4930241 (+) 1704 WP_031668596.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA2_RS22920 (PALA2_04561) pilC 4930245..4931462 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PALA2_RS22925 (PALA2_04562) pilD 4931465..4932337 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA2_RS22930 (PALA2_04563) coaE 4932334..4932945 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PALA2_RS22935 (PALA2_04564) yacG 4932942..4933142 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA2_RS22940 (PALA2_04565) - 4933179..4933388 (-) 210 WP_003094660.1 hypothetical protein -
  PALA2_RS22945 (PALA2_04566) - 4933494..4934183 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA2_RS22950 (PALA2_04567) - 4934180..4934650 (-) 471 WP_003094664.1 hypothetical protein -
  PALA2_RS22955 (PALA2_04568) - 4934647..4935072 (-) 426 WP_003116252.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62653.57 Da        Isoelectric Point: 5.7065

>NTDB_id=732865 PALA2_RS22915 WP_031668596.1 4928538..4930241(+) (pilB) [Pseudomonas aeruginosa strain PALA2]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKRYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=732865 PALA2_RS22915 WP_031668596.1 4928538..4930241(+) (pilB) [Pseudomonas aeruginosa strain PALA2]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCGGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

54.93

100

0.55
  pilF Neisseria gonorrhoeae MS11

50.266

99.295

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.673

94.885

0.49
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.835

95.062

0.483
  pilF Thermus thermophilus HB27

38.407

99.647

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

93.474

0.374