Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P5622_RS11795 Genome accession   NZ_CP120598
Coordinates   2233050..2233667 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis strain PRO115     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2228050..2238667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5622_RS11760 (P5622_11760) aroE 2228708..2229550 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  P5622_RS11765 (P5622_11765) yhbY 2229544..2229834 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  P5622_RS11770 (P5622_11770) nadD 2229846..2230415 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  P5622_RS11775 (P5622_11775) yqeK 2230405..2230965 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P5622_RS11780 (P5622_11780) rsfS 2230983..2231339 (+) 357 WP_003229971.1 ribosome silencing factor -
  P5622_RS11785 (P5622_11785) yqeM 2231336..2232079 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  P5622_RS11790 (P5622_11790) comER 2232145..2232966 (-) 822 WP_004398597.1 late competence protein ComER -
  P5622_RS11795 (P5622_11795) comEA 2233050..2233667 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  P5622_RS11800 (P5622_11800) comEB 2233734..2234303 (+) 570 WP_003229978.1 ComE operon protein 2 -
  P5622_RS11805 (P5622_11805) comEC 2234307..2236637 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P5622_RS11810 (P5622_11810) yqzM 2236677..2236811 (-) 135 WP_003229983.1 YqzM family protein -
  P5622_RS11815 (P5622_11815) - 2236852..2237001 (+) 150 WP_003229985.1 hypothetical protein -
  P5622_RS11820 (P5622_11820) holA 2237041..2238084 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  P5622_RS11825 (P5622_11825) rpsT 2238099..2238365 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=732836 P5622_RS11795 WP_004398514.1 2233050..2233667(+) (comEA) [Bacillus subtilis strain PRO115]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=732836 P5622_RS11795 WP_004398514.1 2233050..2233667(+) (comEA) [Bacillus subtilis strain PRO115]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39