Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   P5628_RS08735 Genome accession   NZ_CP120577
Coordinates   1656522..1658570 (+) Length   682 a.a.
NCBI ID   WP_032725686.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO53     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1651522..1663570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS08705 (P5628_08705) spoVM 1652158..1652238 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  P5628_RS08710 (P5628_08710) rpmB 1652311..1652499 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  P5628_RS08715 (P5628_08715) yloU 1652776..1653138 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  P5628_RS08720 (P5628_08720) yloV 1653154..1654815 (+) 1662 WP_277709587.1 DAK2 domain-containing protein -
  P5628_RS08725 (P5628_08725) sdaAB 1654954..1655616 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  P5628_RS08730 (P5628_08730) sdaAA 1655642..1656544 (+) 903 WP_032725684.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  P5628_RS08735 (P5628_08735) recG 1656522..1658570 (+) 2049 WP_032725686.1 ATP-dependent DNA helicase RecG Machinery gene
  P5628_RS08740 (P5628_08740) fapR 1658679..1659245 (+) 567 WP_003232044.1 transcription factor FapR -
  P5628_RS08745 (P5628_08745) plsX 1659259..1660260 (+) 1002 WP_038828221.1 phosphate acyltransferase PlsX -
  P5628_RS08750 (P5628_08750) fabD 1660279..1661232 (+) 954 WP_015383644.1 ACP S-malonyltransferase -
  P5628_RS08755 (P5628_08755) fabG 1661225..1661965 (+) 741 WP_014476789.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  P5628_RS08760 (P5628_08760) acpP 1662049..1662282 (+) 234 WP_003154310.1 acyl carrier protein -
  P5628_RS08765 (P5628_08765) rncS 1662422..1663171 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78125.62 Da        Isoelectric Point: 6.9755

>NTDB_id=732681 P5628_RS08735 WP_032725686.1 1656522..1658570(+) (recG) [Bacillus subtilis strain PRO53]
MIQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKQLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDQSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=732681 P5628_RS08735 WP_032725686.1 1656522..1658570(+) (recG) [Bacillus subtilis strain PRO53]
GTGATACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGTTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATTACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGCAGCTTTC
GCTCGGCTCTGTGGTGACGGTCTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGATCAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGAGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACAGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACAGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTGGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAC
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.56

100

0.996
  recG/mmsA Streptococcus pneumoniae R6

49.175

97.801

0.481
  recG/mmsA Streptococcus pneumoniae R36A

49.175

97.801

0.481
  recG Neisseria meningitidis strain C311

39.695

96.041

0.381