Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   P5708_RS00290 Genome accession   NZ_CP120563
Coordinates   59475..61556 (-) Length   693 a.a.
NCBI ID   WP_021516576.1    Uniprot ID   -
Organism   Escherichia coli strain H22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 54475..66556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5708_RS00275 (P5708_00275) yicH 54735..56444 (-) 1710 WP_089638541.1 AsmA family protein -
  P5708_RS00280 (P5708_00280) xanP 56565..57956 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  P5708_RS00285 (P5708_00285) gltS 58236..59441 (+) 1206 WP_000468826.1 sodium/glutamate symporter -
  P5708_RS00290 (P5708_00290) recG 59475..61556 (-) 2082 WP_021516576.1 ATP-dependent DNA helicase RecG Machinery gene
  P5708_RS00295 (P5708_00295) trmH 61562..62251 (-) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  P5708_RS00300 (P5708_00300) spoT 62258..64366 (-) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  P5708_RS00305 (P5708_00305) rpoZ 64385..64660 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  P5708_RS00310 (P5708_00310) gmk 64715..65338 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76510.47 Da        Isoelectric Point: 7.3384

>NTDB_id=732367 P5708_RS00290 WP_021516576.1 59475..61556(-) (recG) [Escherichia coli strain H22]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=732367 P5708_RS00290 WP_021516576.1 59475..61556(-) (recG) [Escherichia coli strain H22]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTACAGGATTTGCTCTTACACCTTCCTCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTACAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCATCGCCCACCGCCGACGCTGCAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGTCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCACCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCAATGATGCGCCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGTTGCGTGCGGTTGCCCACGGTAAACAGGTG
GCCCTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTTCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTAGGCTGGCTCGCCGGAAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTACATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCACCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGATATCATTGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTACTGGAAGCGCAGGCGGCGGAAGCGAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGACGGATGAAACCCGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAACTACACCTGCTGGTTGCCACTACTGTCATTGAAGTCGGTGTTGATGTACCC
AATGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGTTACGTGGCCGCGTGGGTCG
TGGCGCGGTGGCCTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATCCGCCTGCAGGTACTAC
GCGACAGCAACGATGGTTTTGTGATTGCACAGAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGGAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

39.074

99.711

0.39
  recG/mmsA Streptococcus pneumoniae R36A

39.074

99.711

0.39
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371