Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N5980_RS07655 Genome accession   NZ_CP104651
Coordinates   1539721..1540917 (+) Length   398 a.a.
NCBI ID   WP_004785573.1    Uniprot ID   N8WSW4
Organism   Acinetobacter variabilis strain 2021CK-01005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1534721..1545917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5980_RS07640 (N5980_07635) - 1535757..1536287 (+) 531 WP_004785566.1 hypothetical protein -
  N5980_RS07645 (N5980_07640) - 1536839..1537888 (+) 1050 WP_004785567.1 3-deoxy-7-phosphoheptulonate synthase -
  N5980_RS07650 (N5980_07645) - 1538143..1539321 (+) 1179 Protein_1477 PilT/PilU family type 4a pilus ATPase -
  N5980_RS07655 (N5980_07650) pilU 1539721..1540917 (+) 1197 WP_004785573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N5980_RS07660 (N5980_07655) - 1540992..1542165 (-) 1174 Protein_1479 MFS transporter -
  N5980_RS07665 (N5980_07660) gcvH 1542405..1542779 (-) 375 WP_004280423.1 glycine cleavage system protein GcvH -
  N5980_RS07670 (N5980_07665) - 1542862..1543890 (-) 1029 WP_004785579.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 44935.37 Da        Isoelectric Point: 6.2770

>NTDB_id=731251 N5980_RS07655 WP_004785573.1 1539721..1540917(+) (pilU) [Acinetobacter variabilis strain 2021CK-01005]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQTLTAEKTKLFAYSLMNDKQSQEFEAELEC
NFAITVPGVSRFRVNVFVQQLQVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMGKS
RELGMQTFDQALFDLYNEGEISYEEALRNADSTNELRLQIKLKSNRQDNPAAGEATQFTMMQEPKAEEKEQENTSASE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=731251 N5980_RS07655 WP_004785573.1 1539721..1540917(+) (pilU) [Acinetobacter variabilis strain 2021CK-01005]
ATGTTCACGGCAGAACTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACCAAAGTAGTGGAATATGGGGGCTC
AGACTTATTTATTACCGCAGATTTTCCACCAAGTATCAAACATCAGGGCTTAATGAAGCCTTTTGGACAACAGACGCTAA
CTGCTGAAAAAACCAAACTGTTTGCCTATAGCTTGATGAATGACAAACAGAGTCAAGAGTTTGAAGCCGAACTAGAATGT
AACTTCGCGATTACCGTGCCGGGTGTATCGCGCTTCCGGGTAAATGTTTTCGTACAACAGCTTCAGGTTGGGATGGTGAT
CCGGACCATTACGGCAGAAATTCCAAATTTTCAGAAACTGAAATTACCAGAATCATTGAAACACGTGATTATGGAAAAAC
GTGGTCTGGTACTGGTTGTGGGAGGAACAGGTTCAGGTAAATCGACCTCTTTGGCAGCGATGATCGACTACCGCAATGAA
AATTCTGCAGGGCATATTATTACCGTGGAAGACCCAGTGGAATATGTGCATAAGCACAAGAAATCTATGATTACTCACCG
TGAAGTCGGGGTAGATTGCCATTCCTGGCACAATGCGTTGAAAAATACCTTGCGTCAGGCACCTGATGTGATTCTGATTG
GTGAGATTCGCGATACTGAAACCATGGAACACGCAATTGCCTTCGCCGAAACCGGACATTTATGTCTGGGCACACTGCAT
TCCAATAATGCTAACCAGACTTTGGACCGGATTATTAACTTCTTCCCGGAAGAACGCCGTAATCAGTTATTGATGGATCT
ATCTTCGAATATGAAGGCAATTATTTCCCAGCGTCTGATTCGTACTCAGGATGGGAAAGGCCGTCGGGCTGCGATTGAAA
TCATGTTAAATACACCTTTGATTTCTGATCTGATTTTAAAAGGTGAGTTTCATGAGCTGAAAGCTATTATGGGCAAATCT
CGTGAATTGGGCATGCAGACTTTCGATCAGGCCTTATTTGACCTGTATAACGAGGGTGAGATTTCTTATGAGGAAGCATT
GCGTAATGCCGATTCGACTAATGAATTACGTTTACAGATCAAGCTGAAAAGCAACCGCCAAGATAATCCTGCTGCCGGAG
AAGCAACACAATTCACGATGATGCAAGAACCGAAAGCTGAAGAAAAAGAACAAGAAAATACTTCAGCATCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8WSW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.92

87.437

0.533
  pilU Acinetobacter baylyi ADP1

55.62

87.186

0.485
  pilU Vibrio cholerae strain A1552

51.781

91.709

0.475