Detailed information NTG0730678

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5O86_RS05865
• Genome accession: NZ_CP104581
• Coordinates: 1293969..1294862 (-)
• Length: 297 a.a.
• NCBI ID: WP_016445609.1
• Uniprot ID: A0A1H3L211
• Organism: Delftia tsuruhatensis strain GD03927
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1288969..1299862

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O86_RS05835 (N5O86_05835)	argJ	1289295..1290527 (+)	1233	WP_046986119.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
N5O86_RS05840 (N5O86_05840)	-	1290693..1291649 (+)	957	WP_046986120.1	ATP-binding protein	-
N5O86_RS05845 (N5O86_05845)	-	1291700..1292206 (+)	507	WP_016445605.1	NUDIX domain-containing protein	-
N5O86_RS05850 (N5O86_05850)	-	1292217..1292453 (-)	237	WP_223275109.1	DNA gyrase inhibitor YacG	-
N5O86_RS05855 (N5O86_05855)	zapD	1292471..1293226 (-)	756	WP_016445607.1	cell division protein ZapD	-
N5O86_RS05860 (N5O86_05860)	coaE	1293316..1293954 (-)	639	WP_250438418.1	dephospho-CoA kinase	-
N5O86_RS05865 (N5O86_05865)	pilD	1293969..1294862 (-)	894	WP_016445609.1	A24 family peptidase	Machinery gene
N5O86_RS05870 (N5O86_05870)	pilC	1294862..1296079 (-)	1218	WP_016445610.1	type II secretion system F family protein	Machinery gene
N5O86_RS05875 (N5O86_05875)	pilB	1296118..1297860 (-)	1743	WP_016445611.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N5O86_RS05885 (N5O86_05885)	-	1298097..1299026 (-)	930	WP_016451025.1	polyprenyl synthetase family protein	-
N5O86_RS05890 (N5O86_05890)	rplU	1299257..1299568 (+)	312	WP_012207284.1	50S ribosomal protein L21	-
N5O86_RS05895 (N5O86_05895)	rpmA	1299584..1299841 (+)	258	WP_012207283.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31842.37 Da
• Isoelectric Point: 6.9135

>NTDB_id=730678 N5O86_RS05865 WP_016445609.1 1293969..1294862(-) (pilD) [Delftia tsuruhatensis strain GD03927]
MVLELWMQAALGGLLGLLVGSFLNVVVHRLPRMMEQQWNAECAQWAQEQSSSSATPSPATAPPPAEAPLSLSRPRSRCPH
CGHAIHWYENIPVLSYLALRGRCSECKASISIRYPLVELACAALFAYCAAQWGITPTGAAWCLFSAALLSLALIDWDTTL
LPDDITLPLLWAGLLSSALHWTAVPLADAVWGAAAGYLSLWLVYWAFKLATGKEGMGYGDFKLFAALGAWFGWQALVPII
LISSVIGAIVGIALKINSRLREGGYVPFGPFLAGAGFAAMVFGPQQMLQSLLAALGL

Nucleotide

• Length: 894 bp

>NTDB_id=730678 N5O86_RS05865 WP_016445609.1 1293969..1294862(-) (pilD) [Delftia tsuruhatensis strain GD03927]
ATGGTTTTGGAACTCTGGATGCAGGCTGCATTGGGCGGCCTGCTGGGCCTGTTGGTTGGCAGCTTTCTCAACGTGGTGGT
GCATAGGCTGCCCCGCATGATGGAACAGCAATGGAATGCGGAATGCGCCCAATGGGCGCAAGAGCAATCTTCATCCTCCG
CCACGCCATCGCCCGCCACAGCGCCGCCGCCTGCCGAGGCGCCCCTGAGCCTGAGCCGCCCCCGCTCGCGCTGCCCGCAT
TGCGGCCATGCCATCCACTGGTACGAGAATATTCCCGTGCTCAGTTATCTGGCGCTGCGAGGCCGGTGCTCCGAATGCAA
GGCGTCGATCAGCATCCGCTATCCGCTGGTGGAATTGGCCTGCGCCGCGCTGTTTGCCTACTGTGCGGCGCAATGGGGCA
TCACGCCCACAGGCGCTGCCTGGTGCCTTTTCAGTGCGGCCTTGCTGAGCCTGGCATTGATCGACTGGGACACCACACTG
CTTCCCGACGACATCACCCTGCCCTTGCTGTGGGCCGGACTGCTGTCGTCGGCGCTGCACTGGACCGCCGTGCCTCTGGC
CGACGCGGTATGGGGCGCTGCAGCGGGCTATCTCTCGCTGTGGCTGGTTTACTGGGCCTTCAAGCTGGCCACCGGCAAGG
AAGGCATGGGCTACGGCGACTTCAAGCTGTTTGCCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCACTCGTGCCCATCATC
CTGATCTCGTCGGTGATTGGCGCCATCGTGGGCATCGCGTTGAAGATCAACAGCCGCCTGCGTGAGGGCGGCTATGTTCC
CTTCGGCCCCTTTCTGGCGGGCGCCGGCTTTGCAGCCATGGTGTTCGGACCGCAGCAGATGCTGCAATCCCTATTGGCGG
CCTTGGGCCTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-131)

• Type IV leader peptidase family (Peptidase_A24) (143-252)

3D structure

• AlphaFold DB: A0A1H3L211

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.59	95.286	0.492
pilD	Vibrio campbellii strain DS40M4	46.416	98.653	0.458
pilD	Acinetobacter nosocomialis M2	44.364	92.593	0.411
pilD	Neisseria gonorrhoeae MS11	45.865	89.562	0.411
pilD	Acinetobacter baumannii D1279779	44	92.593	0.407