Detailed information NTG0072846

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: W01_RS08310
• Genome accession: NZ_AP019547
• Coordinates: 1863974..1864846 (+)
• Length: 290 a.a.
• NCBI ID: WP_173053746.1
• Uniprot ID: A0A455XEA7
• Organism: Candidatus Nitrotoga sp. AM1P
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1858974..1869846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
W01_RS13930 (W01_16180)	-	1859436..1860320 (+)	885	WP_198421271.1	hypothetical protein	-
W01_RS08295 (W01_16190)	-	1860393..1860797 (-)	405	WP_173053739.1	hypothetical protein	-
W01_RS08300 (W01_16200)	-	1860809..1861753 (-)	945	WP_173053741.1	PEP-CTERM sorting domain-containing protein	-
W01_RS08305 (W01_16220)	pilC	1862416..1863633 (+)	1218	WP_173053743.1	type II secretion system F family protein	Machinery gene
W01_RS08310 (W01_16230)	pilD	1863974..1864846 (+)	873	WP_173053746.1	A24 family peptidase	Machinery gene
W01_RS08315 (W01_16240)	coaE	1864843..1865469 (+)	627	WP_173053748.1	dephospho-CoA kinase	-
W01_RS08320 (W01_16250)	zapD	1865536..1866291 (+)	756	WP_173053750.1	cell division protein ZapD	-
W01_RS08325 (W01_16260)	yacG	1866398..1866586 (+)	189	WP_198421394.1	DNA gyrase inhibitor YacG	-
W01_RS08330 (W01_16270)	-	1866574..1867554 (-)	981	WP_242006917.1	Nudix family hydrolase	-
W01_RS08335 (W01_16280)	-	1867659..1868357 (-)	699	WP_173053756.1	SDR family oxidoreductase	-
W01_RS08340 (W01_16290)	-	1868624..1868929 (-)	306	WP_173053758.1	hypothetical protein	-
W01_RS08345 (W01_16300)	-	1869032..1869664 (-)	633	WP_242006918.1	elongation factor-1 alpha	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31615.70 Da
• Isoelectric Point: 6.5859

>NTDB_id=72846 W01_RS08310 WP_173053746.1 1863974..1864846(+) (pilD) [Candidatus Nitrotoga sp. AM1P]
MTALVELLQSLPFFFIVLCGITGLLVGSFLNVVIHRLPKMMEHEWHAQCAELTGNEPANLPPYNLIMPHSACPHCGHKIS
ALENIPVISYLLLRGKCKDCNTPISARYPIVEMVSGLLSAYVAWHFGFGLTAVAALLFIWMLLALTCIDVDTQLLPDDIT
LPLLWLGLLFNLSTTFTDINSAVIGAITGYLALWSIYWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLVIMLSSVVG
AVVGITLIIAARLGRNVPIPFGPYLAGGGVIALFWGQPLTQGYLQLFSIQ

Nucleotide

• Length: 873 bp

>NTDB_id=72846 W01_RS08310 WP_173053746.1 1863974..1864846(+) (pilD) [Candidatus Nitrotoga sp. AM1P]
ATGACAGCACTCGTTGAGTTACTACAAAGCCTTCCGTTTTTTTTCATTGTTCTGTGTGGCATTACCGGTCTATTAGTGGG
CAGTTTTCTAAATGTAGTCATTCACCGCTTACCCAAGATGATGGAGCATGAATGGCATGCCCAATGTGCTGAACTCACCG
GCAATGAACCAGCAAATCTACCGCCTTACAACTTGATCATGCCGCATTCCGCCTGTCCGCATTGTGGCCACAAGATAAGC
GCGCTGGAAAACATCCCGGTCATCAGCTATCTACTGTTACGCGGCAAATGCAAGGATTGCAACACACCTATCTCAGCACG
GTATCCCATCGTGGAAATGGTGTCGGGACTTCTGAGCGCCTATGTCGCATGGCATTTCGGCTTCGGTTTAACTGCCGTGG
CCGCGTTGTTGTTTATCTGGATGCTGCTGGCACTAACCTGCATTGACGTTGATACTCAACTGCTACCAGATGACATCACA
TTGCCCCTTCTGTGGCTCGGCCTACTGTTTAATTTGTCGACCACATTTACTGACATTAATAGTGCCGTCATCGGCGCAAT
CACAGGGTATCTAGCGCTATGGAGCATATATTGGCTATTTAAGCTCGCCACCGGCAAGGAAGGCATGGGTTATGGTGATT
TCAAACTGCTGGCAGCAATCGGCGCATGGCTGGGCTGGCAAATGCTACCGCTGGTTATTATGCTCTCTTCAGTAGTAGGC
GCGGTGGTCGGCATTACATTGATTATCGCGGCGCGTCTTGGTCGCAACGTCCCCATTCCATTTGGCCCGTATTTGGCAGG
TGGCGGCGTGATTGCGTTATTCTGGGGGCAACCCCTTACGCAGGGCTACCTGCAACTATTCAGCATTCAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A455XEA7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.873	97.931	0.528
pilD	Vibrio campbellii strain DS40M4	51.237	97.586	0.5
pilD	Acinetobacter baumannii D1279779	48.387	96.207	0.466
pilD	Acinetobacter nosocomialis M2	47.67	96.207	0.459
pilD	Neisseria gonorrhoeae MS11	48.699	92.759	0.452