Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N2A98_RS02135 Genome accession   NZ_CP104045
Coordinates   486909..488234 (+) Length   441 a.a.
NCBI ID   WP_078466522.1    Uniprot ID   -
Organism   Pseudomonas sp. FJ2-5-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481909..493234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2A98_RS02115 (N2A98_02115) - 481971..484436 (-) 2466 WP_044270721.1 penicillin-binding protein 1A -
  N2A98_RS02120 (N2A98_02120) pilM 484667..485323 (+) 657 WP_044270723.1 type IV pilus assembly protein PilM -
  N2A98_RS02125 (N2A98_02125) - 485320..485871 (+) 552 WP_044270725.1 PilN domain-containing protein -
  N2A98_RS02130 (N2A98_02130) - 485868..486839 (+) 972 WP_275622963.1 pilus assembly protein PilP -
  N2A98_RS02135 (N2A98_02135) pilQ 486909..488234 (+) 1326 WP_078466522.1 type IV pilus secretin PilQ family protein Machinery gene
  N2A98_RS02140 (N2A98_02140) aroK 488239..488757 (+) 519 WP_003187914.1 shikimate kinase AroK -
  N2A98_RS02145 (N2A98_02145) aroB 488884..489984 (+) 1101 WP_078802353.1 3-dehydroquinate synthase -
  N2A98_RS02150 (N2A98_02150) - 489994..491571 (+) 1578 WP_078802354.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47751.68 Da        Isoelectric Point: 4.8066

>NTDB_id=727200 N2A98_RS02135 WP_078466522.1 486909..488234(+) (pilQ) [Pseudomonas sp. FJ2-5-13]
MAAAVPNRLDLIQLPPPDGASSVSYTGAKLTLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLMLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGATDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLTRGGGGVGGIAKPQADGAEPPQ
NLPSSPFVDLGSLAGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=727200 N2A98_RS02135 WP_078466522.1 486909..488234(+) (pilQ) [Pseudomonas sp. FJ2-5-13]
ATGGCTGCTGCTGTGCCCAATCGCCTGGACCTGATCCAACTGCCGCCGCCCGACGGGGCCTCGTCGGTCAGCTATACAGG
CGCCAAACTGACTCTGAACTTCCAGGACATCGACCTGCGCGCGGTATTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGTGATGAGGTGCAGGGCTCGATCACTCTGCGGCTCAAGGATGTGCCCTGGGACCAGGCGCTGGACCTGGTG
CTGCAAACCAAGGGCTTGGACAAGCGGGTCAAGGCGGGCGTGTTGCTGGTGGCGCCCGCCGATGAGTTGGCCGCTCGCGA
GCTGTTGATGCTGGAGTCGCGCAAGCAGATGGCTGAGCTCGCGCCCTTGCGGCGGGAGCTGCTGCAGGTCAATTACGCCA
AGGCCGCCGACCTGGCCAAGCTGTTTCAGTCGGTGACCGGCTTTGAGGGCGCCACCGATGAGCGCGGTTCGGTGGCGGTG
GATGACCGCACCAACAACATCATCGCCTACCAGACTGGCGAGCGGCTCGAAGAACTGCGGCGCATCGTGGCGCAGTTGGA
TATCCCGGTACGCCAGGTCATGATCGAAGCGCGGATTGTGGAGGCCAATGTCGACTACGACAAAAGCCTGGGTGTGCGCT
GGGGCGGGCGGCTCACCCGTGGCGGCGGCGGTGTTGGTGGTATTGCCAAGCCGCAGGCAGACGGCGCCGAGCCGCCGCAA
AACCTGCCCAGTTCACCCTTTGTCGACTTGGGGTCGCTGGCCGGAACCTCGGGCCTGGGGATCGCCTACATCACCGACAA
CCTGTTGCTGGACCTTGAGCTGACCGCCATGGAAAAAACCGGCAACGGTGAAATCGTCTCGCAACCCAAGGTCGTCACCT
CCGACAAAGAGACTGCACGCATCCTCAAAGGCACCGAGATTCCTTACCAGGAATCCACCTCCCAGGGCGCCACCTCGGTG
TCGTTCAAAGAGGCTTCGCTGTCGCTGGAGGTGACGCCGCAAATCACCCCCGACGACCATGTGATCATGGCGGTCAAGGT
CACCAAGGATGAGCCCGACTACCTGAACCGCCTCAATGACGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTTC
TGGTCAGGGATGGCGAGACCATCGTCATTGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(54-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.071

99.546

0.707
  comQ Acinetobacter baylyi ADP1

41.909

100

0.458
  pilQ Acinetobacter baumannii D1279779

40.162

100

0.449
  pilQ Acinetobacter baumannii strain A118

40.162

100

0.449
  pilQ Legionella pneumophila strain Lp02

44.869

95.011

0.426
  pilQ Legionella pneumophila strain ERS1305867

44.869

95.011

0.426
  pilQ Vibrio campbellii strain DS40M4

43.868

96.145

0.422
  pilQ Vibrio cholerae strain A1552

43.124

97.279

0.42
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.124

97.279

0.42