Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   P1K87_RS08305 Genome accession   NZ_CP119596
Coordinates   1640857..1641219 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain MG-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1635857..1646219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1K87_RS08275 (P1K87_08275) flgD 1636742..1637182 (+) 441 WP_007409782.1 flagellar hook assembly protein FlgD -
  P1K87_RS08280 (P1K87_08280) flgG 1637207..1637983 (+) 777 WP_007409783.1 flagellar basal body rod protein FlgG -
  P1K87_RS08285 (P1K87_08285) - 1638023..1638238 (+) 216 WP_003154243.1 flagellar FlbD family protein -
  P1K87_RS08290 (P1K87_08290) fliL 1638235..1638654 (+) 420 WP_032874897.1 flagellar basal body-associated protein FliL -
  P1K87_RS08295 (P1K87_08295) fliM 1638688..1639686 (+) 999 WP_003154240.1 flagellar motor switch protein FliM -
  P1K87_RS08300 (P1K87_08300) fliY 1639676..1640830 (+) 1155 WP_032874899.1 flagellar motor switch phosphatase FliY -
  P1K87_RS08305 (P1K87_08305) pilH 1640857..1641219 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  P1K87_RS08310 (P1K87_08310) fliZ 1641234..1641887 (+) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  P1K87_RS08315 (P1K87_08315) fliP 1641880..1642545 (+) 666 WP_012117556.1 flagellar type III secretion system pore protein FliP -
  P1K87_RS08320 (P1K87_08320) fliQ 1642560..1642829 (+) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  P1K87_RS08325 (P1K87_08325) fliR 1642836..1643615 (+) 780 WP_032874901.1 flagellar biosynthetic protein FliR -
  P1K87_RS08330 (P1K87_08330) flhB 1643615..1644694 (+) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -

Sequence


Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=726863 P1K87_RS08305 WP_003154238.1 1640857..1641219(+) (pilH) [Bacillus amyloliquefaciens strain MG-2]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=726863 P1K87_RS08305 WP_003154238.1 1640857..1641219(+) (pilH) [Bacillus amyloliquefaciens strain MG-2]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTAGTGACAATGG
ATATTACAATGCCTGAAATGGATGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGTCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383