Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   N0B18_RS13515 Genome accession   NZ_CP103995
Coordinates   2501010..2501627 (-) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM117109     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2496010..2506627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B18_RS13485 (N0B18_13485) rpsT 2496311..2496577 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  N0B18_RS13490 (N0B18_13490) holA 2496592..2497635 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  N0B18_RS13495 (N0B18_13495) - 2497675..2497788 (-) 114 WP_122060479.1 hypothetical protein -
  N0B18_RS13500 (N0B18_13500) yqzM 2497865..2497999 (+) 135 WP_003229983.1 YqzM family protein -
  N0B18_RS13505 (N0B18_13505) comEC 2498040..2500370 (-) 2331 WP_046160607.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  N0B18_RS13510 (N0B18_13510) comEB 2500374..2500943 (-) 570 WP_003229978.1 ComE operon protein 2 -
  N0B18_RS13515 (N0B18_13515) comEA 2501010..2501627 (-) 618 WP_046160608.1 competence protein ComEA Machinery gene
  N0B18_RS13520 (N0B18_13520) comER 2501711..2502532 (+) 822 WP_032726195.1 late competence protein ComER -
  N0B18_RS13525 (N0B18_13525) yqeM 2502598..2503341 (-) 744 WP_046160609.1 class I SAM-dependent methyltransferase -
  N0B18_RS13530 (N0B18_13530) rsfS 2503338..2503694 (-) 357 WP_014480315.1 ribosome silencing factor -
  N0B18_RS13535 (N0B18_13535) yqeK 2503712..2504272 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  N0B18_RS13540 (N0B18_13540) nadD 2504262..2504831 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  N0B18_RS13545 (N0B18_13545) yhbY 2504843..2505133 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  N0B18_RS13550 (N0B18_13550) aroE 2505127..2505969 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=726851 N0B18_RS13515 WP_046160608.1 2501010..2501627(-) (comEA) [Bacillus subtilis strain SRCM117109]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=726851 N0B18_RS13515 WP_046160608.1 2501010..2501627(-) (comEA) [Bacillus subtilis strain SRCM117109]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376