Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PY721_RS13885 Genome accession   NZ_CP119008
Coordinates   2805965..2806642 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain CE0011b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2800965..2811642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY721_RS13855 (PY721_13855) istA 2801107..2802129 (+) 1023 WP_000255946.1 IS21-like element IS100 family transposase -
  PY721_RS13860 (PY721_13860) istB 2802126..2802908 (+) 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
  PY721_RS13865 (PY721_13865) gspJ 2802964..2803353 (+) 390 Protein_2722 type II secretion system minor pseudopilin GspJ -
  PY721_RS13870 (PY721_13870) gspK 2803346..2804329 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  PY721_RS13875 (PY721_13875) gspL 2804344..2805507 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  PY721_RS13880 (PY721_13880) gspM 2805504..2805965 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  PY721_RS13885 (PY721_13885) pilD 2805965..2806642 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  PY721_RS13890 (PY721_13890) bfr 2806671..2807147 (-) 477 WP_000675504.1 bacterioferritin -
  PY721_RS13895 (PY721_13895) bfd 2807219..2807413 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  PY721_RS13900 (PY721_13900) chiA 2807582..2810275 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=724644 PY721_RS13885 WP_000178161.1 2805965..2806642(+) (pilD) [Escherichia coli strain CE0011b]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=724644 PY721_RS13885 WP_000178161.1 2805965..2806642(+) (pilD) [Escherichia coli strain CE0011b]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378