Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG/mmsA   Type   Machinery gene
Locus tag   PWP91_RS00745 Genome accession   NZ_CP118738
Coordinates   148883..150883 (-) Length   666 a.a.
NCBI ID   WP_058217278.1    Uniprot ID   -
Organism   Lactococcus lactis strain P-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 143883..155883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWP91_RS00720 (PWP91_00720) - 144352..144966 (+) 615 WP_010906406.1 TetR/AcrR family transcriptional regulator -
  PWP91_RS00725 (PWP91_00725) - 145045..145773 (-) 729 WP_274998976.1 pseudouridine synthase -
  PWP91_RS00730 (PWP91_00730) - 145869..146504 (-) 636 WP_003132036.1 hypothetical protein -
  PWP91_RS00735 (PWP91_00735) mnmE 146717..148084 (+) 1368 WP_003131240.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  PWP91_RS00740 (PWP91_00740) - 148137..148883 (-) 747 WP_274998704.1 hypothetical protein -
  PWP91_RS00745 (PWP91_00745) recG/mmsA 148883..150883 (-) 2001 WP_058217278.1 ATP-dependent DNA helicase RecG Machinery gene
  PWP91_RS00750 (PWP91_00750) - 151021..151365 (-) 345 WP_010906411.1 zinc ribbon domain-containing protein YjdM -
  PWP91_RS00755 (PWP91_00755) gap 151435..152445 (-) 1011 WP_003131244.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  PWP91_RS00760 (PWP91_00760) - 152724..153245 (-) 522 WP_003131246.1 DUF536 domain-containing protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74874.62 Da        Isoelectric Point: 5.8208

>NTDB_id=723518 PWP91_RS00745 WP_058217278.1 148883..150883(-) (recG/mmsA) [Lactococcus lactis strain P-1]
MKLTDSVQFLKGVGPKSVENFHKLGIFTVQDLLLYFPFRYEDFASRSVFELVDGEKATIIGTVVTPANVQYYGFKRNRLS
FKIKEGEAVVAVSFFNQPYLADKVEVGKEIAVYGKWEQAKQQLMGMKVVAQVDDGLEPVYHLTAGMKQSQLVKVIHQIFE
SDLLSELPENLPEFLIKKYRLMNRQEAVLAMHFPEDMEAHKQALRRVKFEELFMFQLKLQALKNKEKSGRAGLEVVFEQG
EIDQKISELPFELTGAQLSALSEILTDMKSPYHMNRLLQGDVGSGKTVVASLAMYAACLANFQAAIMVPTEILARQHFAN
LKALFPELQISLLVSGLKVAERREILADLVSGHTHMVVGTHALIQEGVDFYNLGLVITDEQHRFGVNQRKILREKGQNPD
VLMMTATPIPRTLAITAFGDMDVSIIDELPKGRQPITTRWVKHEQFSEVLSWIKAEVAKDSQVYFISPLIEESETLDLKN
AEALFAELNEYFGLFAKIGLLHGKMKNDEKDQIMQEFKAKKLDILVSTTVIEVGVDVPNATIMVIMDADRFGLSQLHQLR
GRVGRGTKKSYAILVANPKSDSGKQRMKIMTETQNGFILAEEDLKMRGSGEIFGLRQSGLPEFIAADLVNDYNILEVARK
EAVEIFKNADEFPDLIAQVNVDGGFD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=723518 PWP91_RS00745 WP_058217278.1 148883..150883(-) (recG/mmsA) [Lactococcus lactis strain P-1]
ATGAAATTAACAGATTCAGTGCAATTTTTAAAAGGTGTGGGACCGAAATCTGTGGAAAATTTCCATAAGTTAGGAATTTT
CACGGTTCAAGATTTATTGCTTTATTTCCCTTTTCGTTATGAAGATTTCGCAAGTCGTTCGGTTTTTGAACTTGTTGACG
GAGAAAAAGCAACAATTATTGGAACGGTAGTGACACCAGCTAATGTCCAGTATTACGGATTTAAACGTAATCGACTTTCA
TTTAAAATTAAAGAAGGTGAGGCCGTGGTTGCTGTCAGCTTTTTTAATCAACCTTATTTGGCTGACAAAGTGGAAGTTGG
TAAGGAAATTGCAGTTTACGGAAAATGGGAGCAGGCAAAGCAACAACTGATGGGGATGAAAGTTGTGGCTCAGGTGGACG
ATGGCTTAGAACCTGTTTATCATTTGACAGCTGGTATGAAACAAAGTCAATTGGTCAAAGTAATTCATCAGATTTTTGAA
TCAGACCTTCTATCAGAATTACCTGAGAACTTACCAGAATTTTTGATTAAAAAATATCGTCTGATGAACCGGCAAGAAGC
CGTTTTGGCTATGCATTTTCCTGAAGATATGGAAGCTCATAAGCAGGCTTTACGTCGTGTAAAATTTGAAGAATTATTCA
TGTTTCAGTTGAAATTACAGGCGTTAAAAAATAAAGAAAAGTCGGGCCGAGCAGGATTAGAAGTTGTTTTTGAGCAAGGA
GAAATTGATCAGAAGATTTCTGAATTACCTTTTGAACTGACAGGTGCTCAATTAAGCGCTTTGTCAGAGATTCTGACGGA
TATGAAATCTCCTTATCACATGAATCGACTTTTACAAGGTGATGTGGGTTCTGGTAAAACAGTTGTTGCTTCCCTTGCCA
TGTACGCGGCTTGTTTGGCGAATTTTCAAGCAGCAATTATGGTACCAACGGAAATCCTTGCCCGTCAGCATTTTGCAAAC
TTGAAAGCTCTTTTTCCAGAATTGCAAATTTCACTTTTGGTTTCGGGTTTGAAAGTGGCAGAGCGCCGCGAGATTTTGGC
TGATTTAGTTAGTGGTCATACACATATGGTAGTTGGAACTCATGCACTAATTCAAGAAGGGGTCGATTTTTATAATTTGG
GTCTTGTGATTACAGATGAACAACACCGTTTCGGAGTTAATCAGCGAAAAATTTTACGAGAAAAAGGGCAAAATCCAGAT
GTTCTAATGATGACAGCTACACCTATTCCAAGAACGCTTGCGATTACGGCTTTTGGGGATATGGATGTTTCAATTATTGA
TGAACTACCCAAGGGACGTCAACCCATTACTACAAGGTGGGTCAAACATGAACAATTTTCCGAAGTTTTAAGTTGGATAA
AAGCAGAGGTTGCTAAAGATTCACAGGTTTATTTTATTTCACCTTTAATTGAGGAAAGTGAAACGTTGGACCTGAAAAAC
GCAGAAGCTCTTTTTGCGGAATTAAATGAATACTTTGGATTATTTGCCAAAATTGGTCTTTTACATGGAAAAATGAAAAA
TGATGAAAAAGACCAAATCATGCAAGAATTTAAGGCTAAAAAATTAGATATTTTGGTTTCAACAACAGTGATTGAAGTGG
GAGTTGACGTTCCTAATGCTACCATTATGGTGATTATGGATGCTGACCGCTTTGGACTTTCGCAGCTTCACCAATTGCGT
GGTCGTGTTGGTCGGGGAACAAAAAAATCTTATGCCATTTTAGTAGCAAATCCTAAGTCCGATTCAGGAAAACAACGGAT
GAAAATTATGACAGAAACACAAAATGGTTTTATTTTAGCCGAAGAAGATTTGAAAATGCGTGGTTCTGGAGAGATTTTTG
GACTTCGTCAGTCAGGACTTCCAGAATTTATTGCGGCTGATTTAGTCAATGATTATAATATTTTAGAAGTGGCTAGAAAA
GAAGCAGTTGAAATTTTTAAAAATGCAGATGAGTTTCCTGATTTGATTGCTCAAGTTAATGTTGACGGGGGCTTTGATTG
A

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(257-411)

Helicase conserved C-terminal domain (Helicase_C)

(456-566)

RecG wedge domain (RecG_wedge)

(7-157)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(593-651)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG/mmsA Streptococcus pneumoniae R6

66.047

96.847

0.64
  recG/mmsA Streptococcus pneumoniae R36A

66.047

96.847

0.64
  recG Bacillus subtilis subsp. subtilis str. 168

50.698

96.847

0.491
  recG Neisseria meningitidis strain C311

40.181

99.399

0.399