Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EKM72_RS01070 Genome accession   NZ_AP019305
Coordinates   215158..215655 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM9458     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210158..220655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM72_RS01045 gcvPB 210320..211792 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  EKM72_RS01050 gcvPA 211785..213131 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EKM72_RS01055 gcvT 213151..214242 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EKM72_RS01060 - 214401..214925 (-) 525 WP_001015120.1 shikimate kinase -
  EKM72_RS01065 - 214915..215061 (-) 147 WP_001792109.1 hypothetical protein -
  EKM72_RS01070 comGF 215158..215655 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  EKM72_RS01075 comGE 215573..215872 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  EKM72_RS01080 comGD 215859..216305 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EKM72_RS01085 comGC 216283..216594 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EKM72_RS01090 comGB 216608..217678 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EKM72_RS01095 comGA 217650..218624 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EKM72_RS01100 - 218676..219299 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EKM72_RS01105 - 219296..219625 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EKM72_RS01110 - 219625..220611 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=72244 EKM72_RS01070 WP_001788897.1 215158..215655(-) (comGF) [Staphylococcus aureus strain TUM9458]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=72244 EKM72_RS01070 WP_001788897.1 215158..215655(-) (comGF) [Staphylococcus aureus strain TUM9458]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment