Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NX819_RS13125 Genome accession   NZ_CP103351
Coordinates   2554824..2555441 (-) Length   205 a.a.
NCBI ID   WP_259266251.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM115947     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549824..2560441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX819_RS13095 (NX819_13095) rpsT 2550125..2550391 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NX819_RS13100 (NX819_13100) holA 2550406..2551449 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  NX819_RS13105 (NX819_13105) - 2551489..2551638 (-) 150 WP_003229985.1 hypothetical protein -
  NX819_RS13110 (NX819_13110) yqzM 2551679..2551813 (+) 135 WP_003229983.1 YqzM family protein -
  NX819_RS13115 (NX819_13115) comEC 2551854..2554184 (-) 2331 WP_069704087.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NX819_RS13120 (NX819_13120) comEB 2554188..2554757 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NX819_RS13125 (NX819_13125) comEA 2554824..2555441 (-) 618 WP_259266251.1 competence protein ComEA Machinery gene
  NX819_RS13130 (NX819_13130) comER 2555525..2556346 (+) 822 WP_014480313.1 late competence protein ComER -
  NX819_RS13135 (NX819_13135) yqeM 2556412..2557155 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  NX819_RS13140 (NX819_13140) rsfS 2557152..2557508 (-) 357 WP_014480315.1 ribosome silencing factor -
  NX819_RS13145 (NX819_13145) yqeK 2557526..2558086 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NX819_RS13150 (NX819_13150) nadD 2558076..2558645 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NX819_RS13155 (NX819_13155) yhbY 2558657..2558947 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NX819_RS13160 (NX819_13160) aroE 2558941..2559783 (-) 843 WP_041351695.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21797.51 Da        Isoelectric Point: 4.7220

>NTDB_id=722230 NX819_RS13125 WP_259266251.1 2554824..2555441(-) (comEA) [Bacillus subtilis strain SRCM115947]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKVGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=722230 NX819_RS13125 WP_259266251.1 2554824..2555441(-) (comEA) [Bacillus subtilis strain SRCM115947]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGTGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.909

100

0.385