Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NX810_RS13620 Genome accession   NZ_CP103350
Coordinates   2604182..2604799 (-) Length   205 a.a.
NCBI ID   WP_046663622.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM117108     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2599182..2609799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX810_RS13590 (NX810_13590) rpsT 2599484..2599750 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NX810_RS13595 (NX810_13595) holA 2599765..2600808 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  NX810_RS13600 (NX810_13600) - 2600848..2600997 (-) 150 WP_003229985.1 hypothetical protein -
  NX810_RS13605 (NX810_13605) yqzM 2601038..2601172 (+) 135 WP_003229983.1 YqzM family protein -
  NX810_RS13610 (NX810_13610) comEC 2601212..2603542 (-) 2331 WP_046663619.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NX810_RS13615 (NX810_13615) comEB 2603546..2604115 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NX810_RS13620 (NX810_13620) comEA 2604182..2604799 (-) 618 WP_046663622.1 competence protein ComEA Machinery gene
  NX810_RS13625 (NX810_13625) comER 2604883..2605704 (+) 822 WP_032726195.1 late competence protein ComER -
  NX810_RS13630 (NX810_13630) yqeM 2605770..2606513 (-) 744 WP_033884459.1 class I SAM-dependent methyltransferase -
  NX810_RS13635 (NX810_13635) rsfS 2606510..2606866 (-) 357 WP_041333759.1 ribosome silencing factor -
  NX810_RS13640 (NX810_13640) yqeK 2606884..2607444 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NX810_RS13645 (NX810_13645) nadD 2607434..2608003 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NX810_RS13650 (NX810_13650) yhbY 2608015..2608305 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NX810_RS13655 (NX810_13655) aroE 2608299..2609141 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21815.63 Da        Isoelectric Point: 4.9739

>NTDB_id=722151 NX810_RS13620 WP_046663622.1 2604182..2604799(-) (comEA) [Bacillus subtilis strain SRCM117108]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPMKQAVPTETENTVVKQEANNDESSETIVIDIKGAVKHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=722151 NX810_RS13620 WP_046663622.1 2604182..2604799(-) (comEA) [Bacillus subtilis strain SRCM117108]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGATGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGTGAAACAATTGTGATAGACATCAAAGGTGCTGTTAAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACTTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

37.727

100

0.405
  comEA Staphylococcus aureus N315

36.818

100

0.395