Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NW977_RS07300 Genome accession   NZ_CP102953
Coordinates   1544363..1544860 (-) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 32-T-13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1539363..1549860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW977_RS07275 (NW977_07275) gcvPB 1539525..1540997 (-) 1473 WP_123900425.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  NW977_RS07280 (NW977_07280) gcvPA 1540990..1542336 (-) 1347 WP_000019689.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NW977_RS07285 (NW977_07285) gcvT 1542356..1543447 (-) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  NW977_RS07290 (NW977_07290) - 1543606..1544130 (-) 525 WP_001015120.1 shikimate kinase -
  NW977_RS07295 (NW977_07295) - 1544120..1544266 (-) 147 WP_001789879.1 hypothetical protein -
  NW977_RS07300 (NW977_07300) comGF 1544363..1544860 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  NW977_RS07305 (NW977_07305) comGE 1544778..1545077 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  NW977_RS07310 (NW977_07310) comGD 1545064..1545510 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NW977_RS07315 (NW977_07315) comGC 1545488..1545799 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NW977_RS07320 (NW977_07320) comGB 1545813..1546883 (-) 1071 WP_258412557.1 competence type IV pilus assembly protein ComGB Machinery gene
  NW977_RS07325 (NW977_07325) comGA 1546855..1547829 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NW977_RS07330 (NW977_07330) - 1547881..1548504 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NW977_RS07335 (NW977_07335) - 1548501..1548830 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NW977_RS07340 (NW977_07340) - 1548830..1549816 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=720250 NW977_RS07300 WP_029694050.1 1544363..1544860(-) (comGF) [Staphylococcus aureus strain 32-T-13]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=720250 NW977_RS07300 WP_029694050.1 1544363..1544860(-) (comGF) [Staphylococcus aureus strain 32-T-13]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97