Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NWE25_RS12070 Genome accession   NZ_CP102866
Coordinates   2541905..2542975 (-) Length   356 a.a.
NCBI ID   WP_038459186.1    Uniprot ID   -
Organism   Bacillus velezensis strain YS-AT-DS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2536905..2547975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWE25_RS12020 (NWE25_12020) sipW 2536964..2537548 (-) 585 WP_012117977.1 signal peptidase I SipW -
  NWE25_RS12025 (NWE25_12025) tapA 2537520..2538191 (-) 672 WP_038459173.1 amyloid fiber anchoring/assembly protein TapA -
  NWE25_RS12030 (NWE25_12030) - 2538450..2538779 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  NWE25_RS12035 (NWE25_12035) - 2538820..2538999 (-) 180 WP_022552966.1 YqzE family protein -
  NWE25_RS12040 (NWE25_12040) comGG 2539056..2539433 (-) 378 WP_038459177.1 competence type IV pilus minor pilin ComGG Machinery gene
  NWE25_RS12045 (NWE25_12045) comGF 2539434..2539934 (-) 501 WP_228842201.1 competence type IV pilus minor pilin ComGF -
  NWE25_RS12050 (NWE25_12050) comGE 2539843..2540157 (-) 315 WP_038459179.1 competence type IV pilus minor pilin ComGE Machinery gene
  NWE25_RS12055 (NWE25_12055) comGD 2540141..2540578 (-) 438 WP_038459181.1 competence type IV pilus minor pilin ComGD Machinery gene
  NWE25_RS12060 (NWE25_12060) comGC 2540568..2540876 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  NWE25_RS12065 (NWE25_12065) comGB 2540881..2541918 (-) 1038 WP_038459183.1 competence type IV pilus assembly protein ComGB Machinery gene
  NWE25_RS12070 (NWE25_12070) comGA 2541905..2542975 (-) 1071 WP_038459186.1 competence type IV pilus ATPase ComGA Machinery gene
  NWE25_RS12075 (NWE25_12075) - 2543172..2544122 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -
  NWE25_RS12080 (NWE25_12080) - 2544268..2545569 (+) 1302 WP_038459188.1 hemolysin family protein -
  NWE25_RS12085 (NWE25_12085) - 2545607..2546437 (-) 831 WP_038459190.1 STAS domain-containing protein -
  NWE25_RS12095 (NWE25_12095) - 2546643..2547023 (-) 381 WP_038459192.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NWE25_RS12100 (NWE25_12100) - 2547260..2547505 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40528.87 Da        Isoelectric Point: 9.5864

>NTDB_id=719430 NWE25_RS12070 WP_038459186.1 2541905..2542975(-) (comGA) [Bacillus velezensis strain YS-AT-DS1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEQNCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=719430 NWE25_RS12070 WP_038459186.1 2541905..2542975(-) (comGA) [Bacillus velezensis strain YS-AT-DS1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCATATACGGCAAGGGCTTCAGATATACACATTGTACC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGGAAGCCGCAAAACGGGTCATTGTCT
TTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGCAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.607

98.596

0.371