Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   PTA50_RS00275 Genome accession   NZ_CP117714
Coordinates   57568..59649 (-) Length   693 a.a.
NCBI ID   WP_261577865.1    Uniprot ID   -
Organism   Escherichia coli strain PBS4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 52568..64649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTA50_RS00260 (PTA50_00260) xanP 53838..55229 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  PTA50_RS00265 (PTA50_00265) gltS 55509..56714 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  PTA50_RS00270 (PTA50_00270) - 56717..57583 (+) 867 WP_000747329.1 hypothetical protein -
  PTA50_RS00275 (PTA50_00275) recG 57568..59649 (-) 2082 WP_261577865.1 ATP-dependent DNA helicase RecG Machinery gene
  PTA50_RS00280 (PTA50_00280) trmH 59655..60344 (-) 690 WP_001070177.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  PTA50_RS00285 (PTA50_00285) spoT 60351..62459 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  PTA50_RS00290 (PTA50_00290) rpoZ 62478..62753 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  PTA50_RS00295 (PTA50_00295) gmk 62808..63431 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76553.52 Da        Isoelectric Point: 7.4896

>NTDB_id=718580 PTA50_RS00275 WP_261577865.1 57568..59649(-) (recG) [Escherichia coli strain PBS4]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKQTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAKFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=718580 PTA50_RS00275 WP_261577865.1 57568..59649(-) (recG) [Escherichia coli strain PBS4]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGCACGCCAGAATTACAGGAAACGCTCACGCCGGTTTATCCAACAACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCACCGACGCTACAGCTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCACAACCTCAGCATGTTAGCCTTACGT
GCCGGAGCACAGCGTTTTCATGCCCAGCCGCTGAGCGCCAATGATGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCAAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTA
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGAACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGCAAAGCGCGACTGTCTCAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGTCTGGCGCTGGTGATTATCGACGAACAACAT
CGCTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATA
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGGCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTAAGTTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAAGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCCAACGCAT
GA

Domains

Predicted by InterProScan.

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)

RecG wedge domain (RecG_wedge)

(15-171)

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.53

96.97

0.509
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.21

94.949

0.372