Detailed information NTG0717442

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: NUU08_RS05520
• Genome accession: NZ_CP102522
• Coordinates: 1067463..1068785 (-)
• Length: 440 a.a.
• NCBI ID: WP_058221332.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain R-707-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1062463..1073785

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NUU08_RS05490 (NUU08_05485)	-	1063606..1064574 (+)	969	WP_003130649.1	PhoH family protein	-
NUU08_RS05495 (NUU08_05490)	-	1064632..1065111 (+)	480	WP_003130650.1	NUDIX hydrolase	-
NUU08_RS05500 (NUU08_05495)	ybeY	1065263..1065751 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
NUU08_RS05505 (NUU08_05500)	-	1065735..1066190 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
NUU08_RS05510 (NUU08_05505)	-	1066204..1066782 (+)	579	WP_003130655.1	nucleotidyltransferase family protein	-
NUU08_RS05515 (NUU08_05510)	comFC	1066816..1067220 (-)	405	WP_003130657.1	ComF family protein	Machinery gene
NUU08_RS05520 (NUU08_05515)	comFA	1067463..1068785 (-)	1323	WP_058221332.1	DEAD/DEAH box helicase	Machinery gene
NUU08_RS05525 (NUU08_05520)	-	1068842..1069471 (+)	630	WP_058221331.1	YigZ family protein	-
NUU08_RS05530 (NUU08_05525)	-	1069605..1070102 (+)	498	WP_003130662.1	VanZ family protein	-
NUU08_RS05535 (NUU08_05530)	-	1070150..1071169 (+)	1020	WP_003130663.1	DUF475 domain-containing protein	-
NUU08_RS05540 (NUU08_05535)	-	1071279..1072031 (+)	753	WP_010905753.1	bifunctional metallophosphatase/5'-nucleotidase	-
NUU08_RS05545 (NUU08_05540)	glyQ	1072316..1073269 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50201.71 Da
• Isoelectric Point: 10.1065

>NTDB_id=717442 NUU08_RS05520 WP_058221332.1 1067463..1068785(-) (comFA) [Lactococcus lactis strain R-707-1]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=717442 NUU08_RS05520 WP_058221332.1 1067463..1068785(-) (comFA) [Lactococcus lactis strain R-707-1]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-233)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.5	90.909	0.677
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	38.018	98.636	0.375