Detailed information NTG0716198

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NRL37_RS10005
• Genome accession: NZ_CP102328
• Coordinates: 2133935..2135149 (+)
• Length: 404 a.a.
• NCBI ID: WP_268194679.1
• Uniprot ID: -
• Organism: Metapseudomonas otitidis strain BC12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2128935..2140149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NRL37_RS09975 (NRL37_09975)	-	2129071..2129460 (-)	390	WP_044409036.1	GtrA family protein	-
NRL37_RS09980 (NRL37_09980)	-	2129457..2130458 (-)	1002	WP_268194677.1	glycosyltransferase family 2 protein	-
NRL37_RS09985 (NRL37_09985)	-	2130451..2131944 (-)	1494	WP_268194678.1	ArnT family glycosyltransferase	-
NRL37_RS28025	-	2132092..2133396 (-)	1305	WP_160481131.1	methyl-accepting chemotaxis protein	-
NRL37_RS10000 (NRL37_10000)	-	2133514..2133792 (-)	279	WP_044409048.1	peptidylprolyl isomerase	-
NRL37_RS10005 (NRL37_10005)	pilU	2133935..2135149 (+)	1215	WP_268194679.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NRL37_RS10010 (NRL37_10010)	-	2135161..2135829 (-)	669	WP_268194680.1	hypothetical protein	-
NRL37_RS10015 (NRL37_10015)	-	2135963..2136697 (-)	735	WP_268194681.1	substrate-binding periplasmic protein	-
NRL37_RS10020 (NRL37_10020)	-	2136783..2137424 (-)	642	WP_268194682.1	alpha/beta fold hydrolase	-
NRL37_RS10025 (NRL37_10025)	-	2137489..2138280 (-)	792	WP_044409054.1	crotonase/enoyl-CoA hydratase family protein	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44498.91 Da
• Isoelectric Point: 6.5154

>NTDB_id=716198 NRL37_RS10005 WP_268194679.1 2133935..2135149(+) (pilU) [Metapseudomonas otitidis strain BC12]
MDLSSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLSADPLKPGDVARIAESIMDPEQRVEFDRELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYRDGPAPAPTPAPVAAPTPPPAATRTQDAASWGLELKLEELPEETPPEDP
GKHF

Nucleotide

• Length: 1215 bp

>NTDB_id=716198 NRL37_RS10005 WP_268194679.1 2133935..2135149(+) (pilU) [Metapseudomonas otitidis strain BC12]
ATGGATCTCTCTTCGATGCTGAAGGTCCTTGCCTCCCAGGATGGCTCGGATCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGAGCGCCGACCCGCTCAAGCCGGGGGACGTGGCGCGTATCGCCGAGTCGA
TCATGGACCCGGAGCAGCGCGTCGAATTCGACCGTGAGCTGGAGATGAACCTAGCGATCTCGGTGCCCAACGTGGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCCGCTTCGA
GGACCTCAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCTCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTCATTGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAACGCCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACTTCCGACGGCAAGCGCCGCGCGGCGGTGGAGGTGCTGCTGGGCACTTCCACCATCAGCGACCTGATCA
AGCGCGGTGACTTCAGCTCCATCAAGGAGATCATGGAGAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAAGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCTGCCAACAACCTGCGTCTCAA
GCTGAAGCTCTACCGCGACGGCCCGGCTCCCGCACCGACGCCCGCTCCCGTCGCCGCACCCACCCCGCCACCGGCCGCGA
CCCGTACCCAGGACGCAGCCAGCTGGGGGCTGGAGCTGAAGCTGGAGGAGCTGCCCGAGGAAACGCCGCCGGAAGATCCG
GGCAAGCATTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.881	0.488
pilU	Acinetobacter baylyi ADP1	52.925	88.861	0.47
pilU	Vibrio cholerae strain A1552	52.125	87.376	0.455