Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PP477_RS26015 Genome accession   NZ_CP116934
Coordinates   5550902..5551774 (+) Length   290 a.a.
NCBI ID   WP_003094651.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CUVET20-956     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5545902..5556774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PP477_RS25990 (PP477_25975) nadC 5546228..5547076 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PP477_RS26000 (PP477_25985) pilA 5547280..5547744 (-) 465 WP_031630343.1 pilin Machinery gene
  PP477_RS26005 (PP477_25990) pilB 5547974..5549674 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PP477_RS26010 (PP477_25995) pilC 5549678..5550898 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  PP477_RS26015 (PP477_26000) pilD 5550902..5551774 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PP477_RS26020 (PP477_26005) coaE 5551771..5552382 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PP477_RS26025 (PP477_26010) yacG 5552379..5552579 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PP477_RS26030 (PP477_26015) - 5552616..5552825 (-) 210 WP_003094660.1 hypothetical protein -
  PP477_RS26035 (PP477_26020) - 5552931..5553620 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  PP477_RS26040 (PP477_26025) - 5553617..5554087 (-) 471 WP_003094664.1 hypothetical protein -
  PP477_RS26045 (PP477_26030) - 5554084..5554509 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  PP477_RS26050 (PP477_26035) - 5554642..5555271 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PP477_RS26055 (PP477_26040) - 5555268..5555717 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  PP477_RS26060 (PP477_26045) - 5555743..5555913 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31852.89 Da        Isoelectric Point: 7.7068

>NTDB_id=714746 PP477_RS26015 WP_003094651.1 5550902..5551774(+) (pilD) [Pseudomonas aeruginosa strain CUVET20-956]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQANYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=714746 PP477_RS26015 WP_003094651.1 5550902..5551774(+) (pilD) [Pseudomonas aeruginosa strain CUVET20-956]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGAACTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.516

96.897

0.538
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469