Detailed information NTG0713250

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NP434_RS12770
• Genome accession: NZ_CP101936
• Coordinates: 2507363..2507980 (-)
• Length: 205 a.a.
• NCBI ID: WP_029317886.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain BGSC 10A5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2502363..2512980

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP434_RS12740 (NP434_12740)	rpsT	2502665..2502931 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
NP434_RS12745 (NP434_12745)	holA	2502946..2503989 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
NP434_RS12750 (NP434_12750)	-	2504029..2504178 (-)	150	WP_003229985.1	hypothetical protein	-
NP434_RS12755 (NP434_12755)	yqzM	2504219..2504353 (+)	135	WP_003229983.1	YqzM family protein	-
NP434_RS12760 (NP434_12760)	comEC	2504393..2506723 (-)	2331	WP_256682881.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NP434_RS12765 (NP434_12765)	comEB	2506727..2507296 (-)	570	WP_003229978.1	ComE operon protein 2	-
NP434_RS12770 (NP434_12770)	comEA	2507363..2507980 (-)	618	WP_029317886.1	competence protein ComEA	Machinery gene
NP434_RS12775 (NP434_12775)	comER	2508064..2508885 (+)	822	WP_014480313.1	late competence protein ComER	-
NP434_RS12780 (NP434_12780)	yqeM	2508951..2509694 (-)	744	WP_029317885.1	class I SAM-dependent methyltransferase	-
NP434_RS12785 (NP434_12785)	rsfS	2509691..2510047 (-)	357	WP_041333759.1	ribosome silencing factor	-
NP434_RS12790 (NP434_12790)	yqeK	2510065..2510625 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NP434_RS12795 (NP434_12795)	nadD	2510615..2511184 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
NP434_RS12800 (NP434_12800)	yhbY	2511196..2511486 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
NP434_RS12805 (NP434_12805)	aroE	2511480..2512322 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21722.44 Da
• Isoelectric Point: 4.7220

>NTDB_id=713250 NP434_RS12770 WP_029317886.1 2507363..2507980(-) (comEA) [Bacillus subtilis subsp. subtilis strain BGSC 10A5]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=713250 NP434_RS12770 WP_029317886.1 2507363..2507980(-) (comEA) [Bacillus subtilis subsp. subtilis strain BGSC 10A5]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGGCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.024	100	0.99
comEA	Staphylococcus aureus MW2	36.364	100	0.39
comEA	Staphylococcus aureus N315	35.455	100	0.38