Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CHBNTHi_RS04085 Genome accession   NZ_AP018781
Coordinates   800681..801373 (+) Length   230 a.a.
NCBI ID   WP_005666688.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-1 isolate 13H43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795681..806373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHBNTHi_RS04065 (CHBNV1_07830) ampD 796952..797512 (-) 561 WP_075985522.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CHBNTHi_RS04070 (CHBNV1_07840) pilA 797627..798076 (+) 450 WP_005666683.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CHBNTHi_RS04075 (CHBNV1_07850) pilB 798073..799467 (+) 1395 WP_005666685.1 GspE/PulE family protein Machinery gene
  CHBNTHi_RS04080 (CHBNV1_07860) pilC 799464..800684 (+) 1221 WP_005666687.1 type II secretion system F family protein Machinery gene
  CHBNTHi_RS04085 (CHBNV1_07870) pilD 800681..801373 (+) 693 WP_005666688.1 A24 family peptidase Machinery gene
  CHBNTHi_RS04090 (CHBNV1_07880) rho 801428..802690 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  CHBNTHi_RS04095 (CHBNV1_07890) metJ 802938..803255 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CHBNTHi_RS04100 (CHBNV1_07900) cueR 803269..803655 (-) 387 WP_038439225.1 Cu(I)-responsive transcriptional regulator -
  CHBNTHi_RS04105 (CHBNV1_07910) - 803732..803938 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  CHBNTHi_RS04110 (CHBNV1_07920) - 804024..804230 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CHBNTHi_RS04115 (CHBNV1_07930) - 804205..806373 (+) 2169 WP_110437728.1 cation-translocating P-type ATPase -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26779.80 Da        Isoelectric Point: 8.8544

>NTDB_id=71325 CHBNTHi_RS04085 WP_005666688.1 800681..801373(+) (pilD) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
MIYFAMFLLGGIFGIALWFYLSGFITRLQQEIYATYIELFPQNFLSFQPSVSSIQQKKCGHILRYFLSVGVGFIFLQIAF
KDSIFTVWFGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYWY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=71325 CHBNTHi_RS04085 WP_005666688.1 800681..801373(+) (pilD) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
ATGATTTACTTCGCAATGTTTTTATTAGGCGGCATCTTCGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTCCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGTGTTGGGGTAGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGTTCGGACTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGGGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGGCGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTGTTATTAGCTTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCAGTCATCATTTATTTCGTCAAATATTACTGGTATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

90.435

100

0.904
  pilD Haemophilus influenzae Rd KW20

90

100

0.9

Multiple sequence alignment