Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   NP436_RS20010 Genome accession   NZ_CP101932
Coordinates   3727933..3728052 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis subsp. natto strain BGSC 27E1     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3722933..3733052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP436_RS19990 (NP436_19990) albG 3723097..3723798 (+) 702 WP_014481222.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  NP436_RS19995 (NP436_19995) ywhL 3723804..3725180 (-) 1377 WP_014481223.1 YncE family protein -
  NP436_RS20000 (NP436_20000) ywhK 3725219..3726574 (-) 1356 WP_014481224.1 YncE family protein -
  NP436_RS22035 - 3726571..3726801 (-) 231 Protein_3878 hypothetical protein -
  NP436_RS20005 (NP436_20005) rapF 3726804..3727949 (+) 1146 WP_014481226.1 response regulator aspartate phosphatase RapF Regulator
  NP436_RS20010 (NP436_20010) phrF 3727933..3728052 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  NP436_RS20015 (NP436_20015) - 3728150..3728577 (+) 428 Protein_3881 YbaK/EbsC family protein -
  NP436_RS20020 (NP436_20020) speB 3728642..3729514 (-) 873 WP_003227545.1 agmatinase -
  NP436_RS20025 (NP436_20025) speE 3729575..3730405 (-) 831 WP_003227543.1 spermidine synthase -
  NP436_RS20030 (NP436_20030) pbpG 3730607..3732682 (+) 2076 WP_014481227.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=713195 NP436_RS20010 WP_009968329.1 3727933..3728052(+) (phrF) [Bacillus subtilis subsp. natto strain BGSC 27E1]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=713195 NP436_RS20010 WP_009968329.1 3727933..3728052(+) (phrF) [Bacillus subtilis subsp. natto strain BGSC 27E1]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487