Detailed information NTG0713194

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: NP436_RS20005
• Genome accession: NZ_CP101932
• Coordinates: 3726804..3727949 (+)
• Length: 381 a.a.
• NCBI ID: WP_014481226.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. natto strain BGSC 27E1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3721804..3732949

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP436_RS19985 (NP436_19985)	albF	3721820..3723100 (+)	1281	WP_014481221.1	pitrilysin family protein	-
NP436_RS19990 (NP436_19990)	albG	3723097..3723798 (+)	702	WP_014481222.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
NP436_RS19995 (NP436_19995)	ywhL	3723804..3725180 (-)	1377	WP_014481223.1	YncE family protein	-
NP436_RS20000 (NP436_20000)	ywhK	3725219..3726574 (-)	1356	WP_014481224.1	YncE family protein	-
NP436_RS22035	-	3726571..3726801 (-)	231	Protein_3878	hypothetical protein	-
NP436_RS20005 (NP436_20005)	rapF	3726804..3727949 (+)	1146	WP_014481226.1	response regulator aspartate phosphatase RapF	Regulator
NP436_RS20010 (NP436_20010)	phrF	3727933..3728052 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
NP436_RS20015 (NP436_20015)	-	3728150..3728577 (+)	428	Protein_3881	YbaK/EbsC family protein	-
NP436_RS20020 (NP436_20020)	speB	3728642..3729514 (-)	873	WP_003227545.1	agmatinase	-
NP436_RS20025 (NP436_20025)	speE	3729575..3730405 (-)	831	WP_003227543.1	spermidine synthase	-
NP436_RS20030 (NP436_20030)	pbpG	3730607..3732682 (+)	2076	WP_014481227.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45627.12 Da
• Isoelectric Point: 5.1503

>NTDB_id=713194 NP436_RS20005 WP_014481226.1 3726804..3727949(+) (rapF) [Bacillus subtilis subsp. natto strain BGSC 27E1]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=713194 NP436_RS20005 WP_014481226.1 3726804..3727949(+) (rapF) [Bacillus subtilis subsp. natto strain BGSC 27E1]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.738	100	0.997
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572