Detailed information NTG0713172

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NP436_RS13180
• Genome accession: NZ_CP101932
• Coordinates: 2446740..2447357 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. natto strain BGSC 27E1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2441740..2452357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP436_RS13150 (NP436_13150)	rpsT	2442042..2442308 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
NP436_RS13155 (NP436_13155)	holA	2442323..2443366 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
NP436_RS13160 (NP436_13160)	-	2443406..2443555 (-)	150	WP_003229985.1	hypothetical protein	-
NP436_RS13165 (NP436_13165)	yqzM	2443596..2443730 (+)	135	WP_003229983.1	YqzM family protein	-
NP436_RS13170 (NP436_13170)	comEC	2443770..2446100 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NP436_RS13175 (NP436_13175)	comEB	2446104..2446673 (-)	570	WP_003229978.1	ComE operon protein 2	-
NP436_RS13180 (NP436_13180)	comEA	2446740..2447357 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
NP436_RS13185 (NP436_13185)	comER	2447441..2448262 (+)	822	WP_014480313.1	late competence protein ComER	-
NP436_RS13190 (NP436_13190)	yqeM	2448328..2449071 (-)	744	WP_014480314.1	class I SAM-dependent methyltransferase	-
NP436_RS13195 (NP436_13195)	rsfS	2449068..2449424 (-)	357	WP_014480315.1	ribosome silencing factor	-
NP436_RS13200 (NP436_13200)	yqeK	2449442..2450002 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NP436_RS13205 (NP436_13205)	nadD	2449992..2450561 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
NP436_RS13210 (NP436_13210)	yhbY	2450573..2450863 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
NP436_RS13215 (NP436_13215)	aroE	2450857..2451699 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=713172 NP436_RS13180 WP_014480312.1 2446740..2447357(-) (comEA) [Bacillus subtilis subsp. natto strain BGSC 27E1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=713172 NP436_RS13180 WP_014480312.1 2446740..2447357(-) (comEA) [Bacillus subtilis subsp. natto strain BGSC 27E1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39