Detailed information NTG0713027

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: PNF29_RS12055
• Genome accession: NZ_CP116773
• Coordinates: 2253560..2256136 (-)
• Length: 858 a.a.
• NCBI ID: WP_272515564.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM125727
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 2248560..2261136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PNF29_RS12035 (PNF29_12035)	pksA	2249359..2249973 (-)	615	WP_017694907.1	TetR/AcrR family transcriptional regulator	-
PNF29_RS12040 (PNF29_12040)	-	2250094..2250651 (+)	558	WP_017694906.1	hypothetical protein	-
PNF29_RS12045 (PNF29_12045)	ymzD	2250806..2251261 (+)	456	WP_026113607.1	regulatory YrvL family protein	-
PNF29_RS12050 (PNF29_12050)	hexB	2251661..2253544 (-)	1884	WP_272515563.1	DNA mismatch repair endonuclease MutL	Machinery gene
PNF29_RS12055 (PNF29_12055)	hexA	2253560..2256136 (-)	2577	WP_272515564.1	DNA mismatch repair protein MutS	Machinery gene
PNF29_RS12060 (PNF29_12060)	cotE	2256270..2256815 (-)	546	WP_003231833.1	outer spore coat protein CotE	-
PNF29_RS12065 (PNF29_12065)	ymcA	2257069..2257500 (-)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
PNF29_RS12070 (PNF29_12070)	miaB	2257502..2259031 (-)	1530	WP_014664026.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
PNF29_RS12075 (PNF29_12075)	kbl	2259179..2260357 (-)	1179	WP_106073925.1	glycine C-acetyltransferase	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97636.69 Da
• Isoelectric Point: 5.1585

>NTDB_id=713027 PNF29_RS12055 WP_272515564.1 2253560..2256136(-) (hexA) [Bacillus subtilis strain SRCM125727]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFMRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAERIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQQLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYIHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPVQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=713027 PNF29_RS12055 WP_272515564.1 2253560..2256136(-) (hexA) [Bacillus subtilis strain SRCM125727]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTACGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGATG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGCTACAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTGGTTCAGCT
TATTACGCCCGGAACTGTAATGGACGGCAAAGGCATCCATGAATCGGAAAATAACTTTATCGCATCTGTTTCAGCCTGCT
CGAACGGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACGATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTATGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTATACTCAAAGCGTAATCTGGAGCTGACCGAAACGATCCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATCAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTGATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTATATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTCGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTGCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAGAGCGCATTGACCCTTGCGGGGATGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGCCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGCTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAATTTGCATTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCAGAGCGCTACATTACCCCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGCGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTGCAGCAGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGCCACTACACGAAACCGGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGCATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCGAAAAAAGCGGTGCTTCCGATTTTTGATCAA
ATTTTTACGCGAATCGGCGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAACGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATATTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATTAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCGCGCGCTCAAGATATTTTAAAAGAGCTTGAACATTCAGGAAACAAACCGGAAGTGCCGGTGCAGAAACCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

• MutS domain V (MutS_V) (598-785)

• MutS domain II (MutS_II) (125-239)

• MutS domain I (MutS_I) (5-116)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.591	99.65	0.494
mutS	Pseudomonas stutzeri strain ATCC 17587	39.135	100	0.401