Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   PNF29_RS12050 Genome accession   NZ_CP116773
Coordinates   2251661..2253544 (-) Length   627 a.a.
NCBI ID   WP_272515563.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM125727     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2246661..2258544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS12025 (PNF29_12025) - 2247492..2247842 (+) 351 WP_272515562.1 hypothetical protein -
  PNF29_RS12030 (PNF29_12030) - 2247870..2249117 (-) 1248 WP_272515560.1 IS256-like element ISBsu2 family transposase -
  PNF29_RS12035 (PNF29_12035) pksA 2249359..2249973 (-) 615 WP_017694907.1 TetR/AcrR family transcriptional regulator -
  PNF29_RS12040 (PNF29_12040) - 2250094..2250651 (+) 558 WP_017694906.1 hypothetical protein -
  PNF29_RS12045 (PNF29_12045) ymzD 2250806..2251261 (+) 456 WP_026113607.1 regulatory YrvL family protein -
  PNF29_RS12050 (PNF29_12050) hexB 2251661..2253544 (-) 1884 WP_272515563.1 DNA mismatch repair endonuclease MutL Machinery gene
  PNF29_RS12055 (PNF29_12055) hexA 2253560..2256136 (-) 2577 WP_272515564.1 DNA mismatch repair protein MutS Machinery gene
  PNF29_RS12060 (PNF29_12060) cotE 2256270..2256815 (-) 546 WP_003231833.1 outer spore coat protein CotE -
  PNF29_RS12065 (PNF29_12065) ymcA 2257069..2257500 (-) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA Regulator

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 70447.38 Da        Isoelectric Point: 5.8675

>NTDB_id=713026 PNF29_RS12050 WP_272515563.1 2251661..2253544(-) (hexB) [Bacillus subtilis strain SRCM125727]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDADSTVIEIDIEEAGLASIRVLDNGEGMENEDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEITTSTGEGAGTKLVLQGGNIISESRSSSRKGTEIVVSNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHHGKNLLQTNGNGDVRHVLAAIYGTAVAKKMLPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVVNGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHDLIRDG
IKDVFKQQQLIPSTQVPKKSAPAIKNEQQFITFDEKPPEKKVPEKSTAPSYSPMKLSSVVKEPVDAEEKLPPLQFDAPPI
VDQEQTLEVSDVSAEQPETFEQECHEEQPQPASDRVPIMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVG
EVEPEVQDMIVPLTFHYSTNEALIIEQHKQELESVGVFLESFGSNSYIVRCHPAWFPKGEEAELIEEIIQQVLDSKNIDI
KKLREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRSTSDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=713026 PNF29_RS12050 WP_272515563.1 2251661..2253544(-) (hexB) [Bacillus subtilis strain SRCM125727]
GTGGCAAAAGTCATCCAGCTGTCAGATGAGCTTTCAAATAAAATAGCGGCGGGCGAGGTTGTGGAACGGCCCGCCTCAGT
CGTCAAAGAATTGGTGGAAAATGCGATCGACGCTGACAGCACAGTCATTGAAATCGATATTGAGGAAGCAGGCCTTGCAT
CCATTCGGGTCTTGGATAACGGCGAAGGAATGGAGAATGAAGATTGCAAGCGAGCTTTCCGCCGCCACGCAACGAGTAAA
ATAAAAGATGAAAATGATTTATTCAGGGTGAGAACGCTCGGTTTCAGGGGAGAAGCTCTGCCGAGTATCGCGTCAGTCTC
TCATCTGGAGATTACAACAAGCACTGGTGAAGGAGCTGGGACGAAGCTCGTCCTCCAAGGAGGAAACATCATTTCCGAAT
CGCGTTCCTCAAGCAGGAAGGGAACTGAAATTGTCGTTTCCAACCTGTTTTTTAACACACCGGCCCGTTTGAAATATATG
AAAACCGTTCATACAGAGCTTGGAAACATTACAGATGTGGTTAACCGTATTGCTCTGGCGCATCCAGAGGTATCGATCCG
CCTGCGCCATCATGGGAAAAACCTGCTTCAAACGAACGGAAACGGAGATGTGCGCCATGTACTGGCAGCGATTTACGGCA
CGGCTGTCGCCAAAAAAATGCTTCCGCTGCATGTGAGCTCCTTGGATTTTGAAGTAAAGGGATATATCGCCCTCCCAGAG
ATTACACGGGCGTCGAGAAACTATATGTCGTCTGTGGTCAATGGCCGTTACATTAAAAATTTCCCGCTCGTCAAAGCGGT
TCATGAAGGCTATCATACGCTTCTGCCGATCGGACGCCATCCGATTACTTTTATAGAAATTACGATGGACCCGATTTTAG
TCGATGTCAACGTGCATCCATCGAAGCTTGAGGTCCGGCTCAGCAAGGAAACAGAGCTTCATGACTTAATACGTGACGGA
ATTAAAGATGTGTTTAAACAGCAGCAGCTGATTCCTAGTACCCAGGTCCCGAAAAAATCAGCACCTGCCATCAAAAATGA
GCAGCAGTTTATAACCTTTGATGAAAAGCCTCCGGAAAAAAAAGTTCCGGAAAAATCGACTGCGCCATCTTATTCACCGA
TGAAGCTCAGCTCAGTCGTAAAAGAGCCGGTTGATGCGGAGGAAAAATTGCCCCCTCTCCAATTTGATGCTCCTCCTATC
GTTGATCAGGAACAAACCCTTGAGGTGTCCGATGTTTCAGCAGAACAGCCTGAAACATTTGAGCAGGAATGCCATGAGGA
ACAGCCGCAGCCAGCGTCTGATCGGGTTCCAATTATGTACCCGATCGGCCAGATGCACGGGACTTATATATTGGCACAAA
ACGAAAACGGACTGTATATTATCGACCAGCACGCCGCCCAAGAACGTATTAAATATGAGTACTTTCGTGAAAAGGTGGGG
GAGGTTGAGCCTGAGGTGCAGGATATGATTGTACCGCTGACGTTCCACTACTCCACAAATGAGGCTTTGATTATTGAACA
GCACAAACAAGAGCTCGAAAGCGTCGGTGTCTTTTTAGAGTCATTCGGCTCTAACAGTTACATCGTCCGCTGCCACCCGG
CCTGGTTTCCAAAGGGAGAAGAAGCAGAGCTCATAGAAGAAATCATTCAGCAGGTGCTCGACTCCAAAAATATAGATATT
AAAAAACTCCGCGAGGAAGCGGCGATTATGATGAGCTGCAAAGGCTCCATCAAAGCAAATCGCCACCTCAGAAACGACGA
AATCAAAGCGCTTCTGGACGACCTCCGAAGCACATCAGACCCATTTACATGCCCACACGGCCGCCCGATCATCATTCACC
ACTCGACATATGAGATGGAAAAGATGTTCAAACGCGTGATGTAG

Domains

Predicted by InterProScan.

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-324)

MutL C terminal dimerisation domain (MutL_C)

(442-583)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

45.342

100

0.466