Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PMJ84_RS30305 Genome accession   NZ_CP116720
Coordinates   6421683..6422306 (-) Length   207 a.a.
NCBI ID   WP_272226503.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2866     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6416683..6427306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMJ84_RS30285 (PMJ84_30285) aroB 6417278..6418384 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  PMJ84_RS30290 (PMJ84_30290) aroK 6418434..6418952 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PMJ84_RS30295 (PMJ84_30295) pilQ 6418964..6421108 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  PMJ84_RS30300 (PMJ84_30300) comL/pilP 6421162..6421686 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PMJ84_RS30305 (PMJ84_30305) pilO 6421683..6422306 (-) 624 WP_272226503.1 type 4a pilus biogenesis protein PilO Machinery gene
  PMJ84_RS30310 (PMJ84_30310) pilN 6422303..6422899 (-) 597 WP_015647905.1 type 4a pilus biogenesis protein PilN Machinery gene
  PMJ84_RS30315 (PMJ84_30315) pilM 6422899..6423963 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PMJ84_RS30320 (PMJ84_30320) - 6424148..6426616 (+) 2469 WP_025297613.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22913.42 Da        Isoelectric Point: 4.9176

>NTDB_id=712362 PMJ84_RS30305 WP_272226503.1 6421683..6422306(-) (pilO) [Pseudomonas aeruginosa strain 2866]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPAARQKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=712362 PMJ84_RS30305 WP_272226503.1 6421683..6422306(-) (pilO) [Pseudomonas aeruginosa strain 2866]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCGGCAGCACGTCA
AAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.078

93.237

0.42
  pilO Acinetobacter baumannii D1279779

44.503

92.271

0.411
  comO/pilO Acinetobacter baylyi ADP1

42.932

92.271

0.396