Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvA   Type   Machinery gene
Locus tag   POF64_RS10350 Genome accession   NZ_CP116713
Coordinates   2027268..2027858 (-) Length   196 a.a.
NCBI ID   WP_000272490.1    Uniprot ID   Q3JYM8
Organism   Streptococcus agalactiae strain A909 Cas9 AEKOrevertant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2022268..2032858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF64_RS10315 (POF64_10315) - 2022289..2022606 (-) 318 WP_000940931.1 DUF1292 domain-containing protein -
  POF64_RS10320 (POF64_10320) ruvX 2022632..2023051 (-) 420 WP_001222109.1 Holliday junction resolvase RuvX -
  POF64_RS10325 (POF64_10325) - 2023051..2023317 (-) 267 WP_000507056.1 IreB family regulatory phosphoprotein -
  POF64_RS10330 (POF64_10330) spx 2023519..2023917 (-) 399 WP_000591161.1 transcriptional regulator Spx -
  POF64_RS10335 (POF64_10335) recA 2024133..2025272 (-) 1140 WP_001085741.1 recombinase RecA Machinery gene
  POF64_RS10340 (POF64_10340) cinA 2025346..2026605 (-) 1260 WP_001200975.1 competence/damage-inducible protein A Machinery gene
  POF64_RS10345 (POF64_10345) - 2026694..2027245 (-) 552 WP_000817341.1 DNA-3-methyladenine glycosylase I -
  POF64_RS10350 (POF64_10350) ruvA 2027268..2027858 (-) 591 WP_000272490.1 Holliday junction branch migration protein RuvA Machinery gene
  POF64_RS10355 (POF64_10355) - 2027860..2029092 (-) 1233 WP_000657472.1 MFS transporter -
  POF64_RS10360 (POF64_10360) hexB 2029124..2031097 (-) 1974 WP_000034614.1 DNA mismatch repair endonuclease MutL Machinery gene
  POF64_RS10365 (POF64_10365) - 2031323..2031526 (+) 204 WP_000191849.1 cold shock domain-containing protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 21696.94 Da        Isoelectric Point: 5.7488

>NTDB_id=711835 POF64_RS10350 WP_000272490.1 2027268..2027858(-) (ruvA) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEVTIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGI
GPTTALAIIAVDDNEGLVSAIDNSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=711835 POF64_RS10350 WP_000272490.1 2027268..2027858(-) (ruvA) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
ATGTACGATTATATTAAAGGAAAATTGAGTAAAATAACAGCTAAATTTATTGTTGTGGAAACAGCTGGATTGGGATACGT
GATATATGTGGCAAATCCCTACTCTTTTTCAGGTTACGTTAATCAAGAAGTTACCATCTACTTACATCAAGTCATTCGCG
ATGATGCACATCTTTTATTTGGTTTTCATACGGAAAATGAAAAAGAAATTTTTCTTAATTTAATTTCAGTATCAGGAATT
GGTCCAACGACCGCTTTAGCAATTATTGCAGTCGATGACAATGAAGGTCTCGTAAGTGCCATTGATAATAGTGATATTAA
GTATTTGACTAAATTTCCAAAGATAGGAAAAAAAACAGCACAACAGATGATCCTTGATTTGTCAGGGAAGTTTGTAGAAG
CTTCTGGTGAGAGTGCGACTAGTCGTAAGGTTAGCTCTGAGCAGAATAGCAATCTTGAAGAAGCTATGGAAGCTTTGTTA
GCGTTGGGGTATAAGGCGACTGAATTGAAAAAAGTAAAAGCCTTTTTTGAAGGTACGAACGAGACTGTGGAGCAATACAT
CAAATCTAGTTTGAAGATGTTGATGAAATAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix domain (HHH_5)

(71-127)

RuvA N terminal domain (RuvA_N)

(1-61)

RuvA, C-terminal domain (RuvA_C)

(151-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3JYM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae R6

67.513

100

0.679
  ruvA Streptococcus pneumoniae D39

67.513

100

0.679
  ruvA Streptococcus pneumoniae TIGR4

67.005

100

0.673
  ruvA Bacillus subtilis subsp. subtilis str. 168

39.409

100

0.408