Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvA   Type   Machinery gene
Locus tag   POF63_RS09840 Genome accession   NZ_CP116708
Coordinates   1929810..1930400 (-) Length   196 a.a.
NCBI ID   WP_000272490.1    Uniprot ID   Q3JYM8
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1924810..1935400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS09805 (POF63_09805) - 1924831..1925148 (-) 318 WP_000940933.1 DUF1292 domain-containing protein -
  POF63_RS09810 (POF63_09810) ruvX 1925174..1925593 (-) 420 WP_001222109.1 Holliday junction resolvase RuvX -
  POF63_RS09815 (POF63_09815) - 1925593..1925859 (-) 267 WP_000507056.1 IreB family regulatory phosphoprotein -
  POF63_RS09820 (POF63_09820) spx 1926061..1926459 (-) 399 WP_000591162.1 transcriptional regulator Spx -
  POF63_RS09825 (POF63_09825) recA 1926675..1927814 (-) 1140 WP_001085742.1 recombinase RecA Machinery gene
  POF63_RS09830 (POF63_09830) cinA 1927888..1929147 (-) 1260 WP_001200976.1 competence/damage-inducible protein A Machinery gene
  POF63_RS09835 (POF63_09835) - 1929236..1929787 (-) 552 WP_000817341.1 DNA-3-methyladenine glycosylase I -
  POF63_RS09840 (POF63_09840) ruvA 1929810..1930400 (-) 591 WP_000272490.1 Holliday junction branch migration protein RuvA Machinery gene
  POF63_RS09845 (POF63_09845) - 1930402..1931634 (-) 1233 WP_000657482.1 MFS transporter -
  POF63_RS09850 (POF63_09850) hexB 1931666..1933639 (-) 1974 WP_000034611.1 DNA mismatch repair endonuclease MutL Machinery gene
  POF63_RS09855 (POF63_09855) - 1933864..1934067 (+) 204 WP_000191849.1 cold shock domain-containing protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 21696.94 Da        Isoelectric Point: 5.7488

>NTDB_id=711297 POF63_RS09840 WP_000272490.1 1929810..1930400(-) (ruvA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEVTIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGI
GPTTALAIIAVDDNEGLVSAIDNSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=711297 POF63_RS09840 WP_000272490.1 1929810..1930400(-) (ruvA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGTACGATTATATTAAAGGAAAATTGAGTAAAATAACAGCTAAATTTATTGTTGTGGAAACAGCTGGATTGGGATACGT
GATATATGTGGCAAATCCCTACTCTTTTTCAGGTTACGTTAATCAAGAAGTTACCATCTACTTACATCAAGTCATTCGCG
ATGATGCACATCTTTTATTTGGTTTTCATACGGAAAATGAAAAAGAAATTTTTCTTAATTTAATTTCAGTATCAGGAATT
GGTCCAACGACCGCTTTAGCAATTATTGCAGTCGATGACAATGAAGGTCTCGTAAGTGCCATTGATAATAGTGATATTAA
GTATTTGACTAAATTTCCAAAGATAGGAAAAAAAACAGCACAACAGATGATCCTTGATTTGTCAGGGAAGTTTGTAGAAG
CTTCTGGTGAGAGTGCGACTAGTCGTAAGGTTAGCTCTGAGCAGAATAGCAATCTTGAAGAAGCTATGGAAGCTTTGTTA
GCGTTGGGGTATAAGGCGACTGAATTGAAAAAAGTAAAAGCCTTTTTTGAAGGTACGAACGAGACTGTGGAGCAATACAT
CAAATCTAGTTTGAAGATGTTGATGAAATAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix domain (HHH_5)

(71-127)

RuvA N terminal domain (RuvA_N)

(1-61)

RuvA, C-terminal domain (RuvA_C)

(151-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3JYM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae R6

67.513

100

0.679
  ruvA Streptococcus pneumoniae D39

67.513

100

0.679
  ruvA Streptococcus pneumoniae TIGR4

67.005

100

0.673
  ruvA Bacillus subtilis subsp. subtilis str. 168

39.409

100

0.408