Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG/mmsA   Type   Machinery gene
Locus tag   POF63_RS08250 Genome accession   NZ_CP116708
Coordinates   1603879..1605894 (-) Length   671 a.a.
NCBI ID   WP_000926633.1    Uniprot ID   Q8DY12
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1598879..1610894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS08230 (POF63_08230) - 1599250..1600632 (-) 1383 WP_000974899.1 HAD-IIB family hydrolase -
  POF63_RS08235 (POF63_08235) - 1600701..1601663 (+) 963 WP_000723059.1 asparaginase domain-containing protein -
  POF63_RS08240 (POF63_08240) aroE 1601698..1602576 (-) 879 WP_001086864.1 shikimate dehydrogenase -
  POF63_RS08245 (POF63_08245) - 1602674..1603588 (-) 915 WP_000939996.1 aldo/keto reductase -
  POF63_RS08250 (POF63_08250) recG/mmsA 1603879..1605894 (-) 2016 WP_000926633.1 ATP-dependent DNA helicase RecG Machinery gene
  POF63_RS08255 (POF63_08255) - 1605974..1607512 (-) 1539 WP_000043255.1 CHAP domain-containing protein -
  POF63_RS08260 (POF63_08260) alr 1607605..1608705 (-) 1101 WP_000625941.1 alanine racemase -
  POF63_RS08265 (POF63_08265) acpS 1608702..1609061 (-) 360 WP_000635015.1 holo-ACP synthase -
  POF63_RS08270 (POF63_08270) - 1609086..1610093 (-) 1008 WP_000505724.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75222.76 Da        Isoelectric Point: 6.7517

>NTDB_id=711280 POF63_RS08250 WP_000926633.1 1603879..1605894(-) (recG/mmsA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFKSKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLS
FKLRQGEAVLNVSFFNQPYLADKIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
ISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMNLQVLKSENKSETNGLPILYSKH
AMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNRLLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYIS
LQELFPDLSIAILTSGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIFREKGENPD
VLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVLEWVKGELQKDAQVYVISPLIEESEALDLKN
AVALHAELSTYFEGIAKVALVHGRMKNDEKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLR
GRVGRGYKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQVADIVEDYPILEEARR
VASDIVKDNNWKENTEWALILDNLRQHSDFD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=711280 POF63_RS08250 WP_000926633.1 1603879..1605894(-) (recG/mmsA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGCTATTACAATCACCAATTTCTAATCTTAAGGGATTTGGACCAAAATCAGCAGAAAAATTTCAGAAATTAGATATTTA
TACAGTAGAAGATTTACTTCTTTATTATCCGTTTCGCTATGAAGATTTTAAATCAAAATCTGTTTTTGATTTAGTGGATG
GTGAAAAAGCAGTCATTACAGGCCTAGTCGTTACTCCAGCTAATGTACAATATTATGGTTTTAAACGTAACCGTTTAAGT
TTTAAGTTGCGTCAAGGGGAAGCTGTCTTAAATGTTAGTTTTTTTAATCAACCCTATTTAGCTGATAAAATAGAACTTGG
TCAAGAGGTAGCTGTCTTTGGTAAATGGGATGCAACTAAATCAGCTATTACTGGGATGAAGGTTTTAGCTCAAGTTGAAG
ATGACATGCAACCTGTTTATCGCGTAGCTCAGGGAATTTCACAGTCTACTTTGATTAAAGCTATTAAGTCAGCTTTTGAA
ATCAGTGCGCATTTGGAATTGAAGGAAAATTTACCAGCTACTTTATTGGAAAAATACCGATTGATGGGTCGTAGTCAGGC
TTGTTTAGCTATGCATTTCCCAAAAGATATCACAGAGTATAAGCAAGCGCTCCGTCGGATTAAATTTGAAGAATTATTTT
ACTTTCAAATGAACCTTCAAGTTTTGAAATCCGAAAATAAATCTGAAACAAATGGTTTGCCTATTCTTTATAGTAAACAT
GCTATGGAGACAAAAATTTCCTCTTTACCTTTTATTCTAACGAATGCTCAAAAGCGCTCTTTAGATGAAATATTATCTGA
TATGTCATCGGGAGCTCATATGAATCGTTTATTGCAAGGAGATGTAGGATCAGGAAAGACAGTCATTGCTGGTCTATCAA
TGTATGCAGCTTATACAGCAGGTTTTCAATCGGCTTTGATGGTTCCAACGGAAATCCTAGCTGAACAACACTACATTAGT
CTGCAAGAGTTATTTCCAGATTTATCAATCGCTATATTAACTTCGGGTATGAAAGCAGCTGTTAAGCGTACGGTTTTAGC
AGCTATTGCAAATGGCTCGGTTGATATGATTGTAGGAACTCATGCTCTTATCCAGGACTCGGTACAGTACCATAAACTGG
GGCTTGTAATTACAGACGAGCAACATCGTTTTGGTGTTAAACAGCGTAGAATTTTCCGTGAAAAGGGAGAAAATCCTGAT
GTTTTAATGATGACAGCCACCCCAATTCCCCGAACTCTAGCAATCACAGCTTTTGGAGAAATGGATGTTTCTATTATTGA
TGAATTACCTGCCGGTCGTAAACCTATTATTACACGCTGGGTGAAACACGAGCAGCTAGGTACTGTGTTGGAATGGGTAA
AAGGTGAATTGCAAAAAGATGCTCAAGTGTATGTCATTTCACCGTTGATTGAAGAGTCAGAAGCTTTAGATTTAAAGAAT
GCAGTAGCATTGCATGCTGAATTATCTACTTATTTTGAAGGAATTGCTAAGGTTGCTCTTGTACATGGACGTATGAAAAA
TGATGAAAAAGATGCTATAATGCAAGATTTCAAGGATAAAAAAAGTCATATTTTAGTATCTACAACAGTTATTGAAGTAG
GGGTAAATGTCCCAAATGCAACGATCATGATTATTATGGATGCCGATCGTTTTGGATTAAGCCAGCTACATCAACTTCGT
GGGCGTGTTGGTCGTGGATATAAACAATCATACGCTGTTTTAGTGGCTAATCCCAAAACTGATTCTGGGAAAAAACGAAT
GACAATCATGACAGAAACGACAGATGGTTTCGTTTTAGCTGAGTCGGATTTAAAAATGCGTGGTTCTGGTGAAATCTTTG
GTACTCGTCAGTCTGGAATTCCAGAATTTCAAGTAGCTGATATCGTTGAGGATTATCCTATTTTAGAAGAAGCCAGACGT
GTTGCTAGTGATATTGTCAAAGATAATAATTGGAAAGAAAATACAGAATGGGCACTTATTCTAGATAATTTACGCCAACA
TTCAGATTTTGATTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(254-411)

Helicase conserved C-terminal domain (Helicase_C)

(458-566)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(593-666)

RecG wedge domain (RecG_wedge)

(7-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DY12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG/mmsA Streptococcus pneumoniae R6

71.982

100

0.72
  recG/mmsA Streptococcus pneumoniae R36A

71.982

100

0.72
  recG Bacillus subtilis subsp. subtilis str. 168

50.225

99.404

0.499
  recG Neisseria meningitidis strain C311

39.117

97.914

0.383