Detailed information    

insolico Bioinformatically predicted

Overview

Name   lytF   Type   Regulator
Locus tag   POF65_RS09065 Genome accession   NZ_CP116706
Coordinates   1776784..1777293 (-) Length   169 a.a.
NCBI ID   WP_000392202.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   cell lysis (predicted from homology)   
Cell lysis

Genomic Context

Location: 1771784..1782293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS09040 (POF65_09040) tsaD 1772293..1773303 (-) 1011 WP_000655088.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  POF65_RS09045 (POF65_09045) rimI 1773379..1773786 (-) 408 WP_000445943.1 ribosomal protein S18-alanine N-acetyltransferase -
  POF65_RS09050 (POF65_09050) tsaB 1773788..1774480 (-) 693 WP_000978606.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  POF65_RS09055 (POF65_09055) - 1774659..1774889 (+) 231 WP_000639570.1 DNA-dependent RNA polymerase subunit epsilon -
  POF65_RS09060 (POF65_09060) - 1774943..1776622 (+) 1680 WP_000065496.1 ribonuclease J -
  POF65_RS09065 (POF65_09065) lytF 1776784..1777293 (-) 510 WP_000392202.1 CHAP domain-containing protein Regulator
  POF65_RS09070 (POF65_09070) glnA 1777441..1778787 (-) 1347 WP_000156225.1 type I glutamate--ammonia ligase -
  POF65_RS09075 (POF65_09075) - 1778821..1779192 (-) 372 WP_000664338.1 MerR family transcriptional regulator -
  POF65_RS09080 (POF65_09080) - 1779272..1779811 (-) 540 WP_000854121.1 aromatic acid exporter family protein -
  POF65_RS09085 (POF65_09085) - 1780074..1781270 (-) 1197 WP_001096753.1 phosphoglycerate kinase -
  POF65_RS09090 (POF65_09090) - 1781405..1782274 (-) 870 WP_000858929.1 5'-nucleotidase, lipoprotein e(P4) family -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 18332.26 Da        Isoelectric Point: 4.4055

>NTDB_id=711071 POF65_RS09065 WP_000392202.1 1776784..1777293(-) (lytF) [Streptococcus agalactiae strain A909 sCas9]
MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVDYLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGN
WWGNGGDWAASAASAGYTVGTQPRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
GVVSYIYPN

Nucleotide


Download         Length: 510 bp        

>NTDB_id=711071 POF65_RS09065 WP_000392202.1 1776784..1777293(-) (lytF) [Streptococcus agalactiae strain A909 sCas9]
ATGGAGGAAAACATGAACATAAAACAATTAAAGTCTAAAACAATGCTTGGAACAGTGGCTTTGGTGTCCGCTTTTTCATT
TGCTTCAACAAATGCAGATGCGAATACATATAACTATGCAGTAGATGTAGACTACCTAGCAAGTGCGGAGGAAATTGCTC
AAGCACATCCAGCTAGTAATACGTTTCCACTAGGTCAATGCACATGGGGTGTAAAGGAGATGGCAACATGGGCAGGTAAC
TGGTGGGGAAACGGTGGTGATTGGGCTGCGAGCGCCGCATCAGCTGGTTATACAGTAGGAACACAACCTCGCGTGGGTTC
TATCGTATGTTGGACTGATGGTAGTTATGGACATGTGGCTTATGTTACAGCTGTAGATCCTGTCACTAATAAAATTCAGG
TTTTAGAATCTAATTATGCAGGTCATCAATGGATTGATAATTATCGTGGTTGGTTTGATCCACAAAATACAGTAACACCA
GGGGTAGTTAGCTACATTTATCCGAATTAA

Domains

Predicted by InterProScan.

CHAP domain (CHAP)

(60-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lytF Streptococcus gordonii strain NCTC7865

57.576

78.107

0.45
  lytF Streptococcus gordonii str. Challis substr. CH1

57.576

78.107

0.45
  SMU.836 Streptococcus mutans UA159

55.118

75.148

0.414