Detailed information    

experimental Experimentally validated

Overview

Name   SMU.836   Type   Unclear
Locus tag   SMU_RS03875 Genome accession   NC_004350
Coordinates   785626..787260 (+) Length   544 a.a.
NCBI ID   WP_002261986.1    Uniprot ID   Q8DUR7
Organism   Streptococcus mutans UA159     
Function   require for competence   
Unclear

Function

Deletion of SMU.836 reduced transformation of both plasmid and chromosomal DNA to <3%. Homology searches suggested that Smu.836 belongs to a family of competence-induced peptidoglycan hydrolases with a conserved enzyme domain and a species-variable cell-binding domain for which the best characterized member is the choline-binding protein D, CbpD, of S. pneumoniae.


Genomic Context

Location: 780626..792260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS03860 (SMU.833) - 781479..782405 (+) 927 WP_002261983.1 glycosyltransferase family 2 protein -
  SMU_RS03865 (SMU.834) - 782537..783475 (+) 939 WP_002261984.1 glycosyltransferase family 2 protein -
  SMU_RS03870 (SMU.835) - 783609..785420 (+) 1812 WP_002261985.1 hypothetical protein -
  SMU_RS03875 (SMU.836) SMU.836 785626..787260 (+) 1635 WP_002261986.1 GBS Bsp-like repeat-containing protein Unclear
  SMU_RS03880 (SMU.837) - 787379..788221 (+) 843 WP_002261987.1 aldo/keto reductase -
  SMU_RS03885 (SMU.838) gorA 788354..789706 (+) 1353 WP_002261988.1 glutathione-disulfide reductase -
  SMU_RS03890 (SMU.839) - 789726..790982 (-) 1257 WP_002261989.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -
  SMU_RS03895 (SMU.840c) - 791322..791768 (-) 447 WP_002261990.1 DUF6556 family protein -

Sequence

Protein


Download         Length: 544 a.a.        Molecular weight: 60286.00 Da        Isoelectric Point: 6.7570

>NTDB_id=411 SMU_RS03875 WP_002261986.1 785626..787260(+) (SMU.836) [Streptococcus mutans UA159]
MRKLKVALFASSILGMLAVSSYTAADTEDNQVTISHYNEQAGTFDVNAVQAANGKTIQSIDVAIWSEENGQDDLKWYHAS
NDGSNQLTVHFNAENHGSKVGSYIAHAYITYTDGNRVGVNLGKRKLSLSAPQLSLKQGGLQLFSKLKPSAADQLFSAVWS
DENGQDDLHWYTADADGNTLAGYANHKGYGTYHVHTYLKQNGKMIPISAQDIDIPKPKVKIQIDKINDTSYDVVVNNVPP
YISSVAIPVWSEQNGQDDLKWYQATKVADGIFKTTVYLKTHRFELGNYQAHIYGDSQLSKKLDGLGETHFNVPSIINYED
PQVTIDHYNINKGTFDVTVAETDNSKAIQSISAAVWSDANQANLYWYEAKQLANGKAAITVDVQKHGNQTGSYNVHVYVH
YNDGTTSGHVLANQQLNQIVHYQPSAVRITAYMNEKNTYPVGQCTWGVKELAPWIPNWLGNGGQWASTVAVKGFKIGTVP
KVGAIACWSDGGYGHVAYVTHVESNNRIQVKEANYKNQQYISNFRGWFDPTTSYLGRLTYIYPD

Nucleotide


Download         Length: 1635 bp        

>NTDB_id=411 SMU_RS03875 WP_002261986.1 785626..787260(+) (SMU.836) [Streptococcus mutans UA159]
ATGAGAAAACTTAAAGTGGCACTTTTTGCAAGCAGTATTTTAGGAATGCTGGCTGTTAGTTCTTATACGGCAGCAGATAC
AGAGGATAATCAGGTAACGATTAGCCATTATAATGAACAGGCTGGAACTTTTGATGTCAACGCTGTACAGGCAGCCAACG
GAAAAACTATTCAATCGATAGATGTTGCGATTTGGTCTGAAGAAAATGGGCAGGATGATTTGAAATGGTATCATGCCAGT
AATGATGGCAGCAATCAATTGACAGTTCATTTTAATGCTGAGAATCATGGCAGTAAGGTAGGTTCTTATATTGCGCATGC
TTATATTACCTATACAGATGGTAATCGAGTCGGGGTTAATTTGGGAAAACGAAAATTATCCTTATCTGCACCGCAATTAT
CCTTAAAACAAGGTGGCCTTCAACTATTTTCTAAGCTGAAACCTAGTGCAGCGGATCAACTTTTTTCAGCAGTTTGGTCG
GATGAGAATGGTCAAGATGATCTTCATTGGTACACGGCAGATGCTGACGGGAATACTTTGGCTGGCTATGCTAATCATAA
AGGTTATGGAACTTACCATGTTCATACTTACCTTAAGCAAAATGGTAAGATGATACCAATTAGTGCTCAAGATATTGATA
TTCCTAAACCGAAAGTCAAGATTCAGATTGATAAAATAAATGATACCAGTTATGATGTTGTTGTTAATAATGTCCCCCCT
TATATTAGTTCAGTAGCCATTCCTGTGTGGAGTGAACAAAATGGCCAAGACGATTTGAAATGGTATCAGGCAACAAAAGT
GGCTGATGGTATATTTAAAACAACTGTTTATTTAAAGACACATCGTTTTGAATTAGGCAACTATCAAGCTCATATTTATG
GCGATAGCCAATTAAGCAAGAAACTGGATGGTTTAGGAGAAACTCATTTTAATGTTCCGTCTATTATTAACTATGAAGAT
CCTCAGGTAACTATTGATCATTATAATATTAACAAAGGAACGTTTGATGTGACTGTAGCTGAAACAGATAATTCAAAAGC
GATACAATCAATCAGTGCTGCTGTTTGGTCTGATGCTAACCAAGCTAATCTTTATTGGTATGAAGCTAAACAGCTAGCAA
ATGGAAAGGCTGCAATTACTGTCGATGTTCAAAAGCATGGCAATCAAACAGGAAGCTACAATGTCCATGTTTATGTTCAT
TATAATGATGGCACGACTAGCGGACATGTTTTGGCTAATCAGCAGCTCAATCAAATTGTCCATTATCAACCTTCTGCAGT
AAGAATAACAGCCTATATGAATGAAAAAAATACTTATCCAGTTGGTCAGTGTACTTGGGGAGTGAAAGAATTAGCTCCTT
GGATACCTAATTGGCTTGGCAATGGCGGGCAGTGGGCAAGTACTGTAGCTGTTAAGGGATTCAAAATAGGAACTGTTCCT
AAAGTTGGTGCTATTGCTTGTTGGAGTGATGGTGGTTATGGCCATGTTGCTTACGTTACCCACGTTGAGAGTAATAACCG
TATTCAAGTGAAAGAAGCTAATTATAAGAATCAACAATATATTTCCAATTTTCGCGGATGGTTTGATCCCACGACTTCCT
ATTTGGGAAGATTAACTTATATTTATCCTGACTAA

Domains

Predicted by InterproScan.

CHAP domain (CHAP)

(436-514)

GBS Bsp-like repeat (GBS_Bsp-like)

(221-309)

GBS Bsp-like repeat (GBS_Bsp-like)

(34-121)

GBS Bsp-like repeat (GBS_Bsp-like)

(152-207)

GBS Bsp-like repeat (GBS_Bsp-like)

(325-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DUR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] R E Eaton et al. (2010) Deletion of competence-induced genes over-expressed in biofilms caused transformation deficiencies in Streptococcus mutans. Molecular Oral Microbiology 25(6):406-17. [PMID: 21040514]