Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   PIB33_RS08475 Genome accession   NZ_CP116391
Coordinates   1624068..1626116 (+) Length   682 a.a.
NCBI ID   WP_032721534.1    Uniprot ID   -
Organism   Bacillus subtilis strain GXD-20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1619068..1631116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS08445 (PIB33_08445) spoVM 1619704..1619784 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  PIB33_RS08450 (PIB33_08450) rpmB 1619857..1620045 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  PIB33_RS08455 (PIB33_08455) yloU 1620322..1620684 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  PIB33_RS08460 (PIB33_08460) fakA 1620700..1622361 (+) 1662 WP_003232052.1 DAK2 domain-containing protein -
  PIB33_RS08465 (PIB33_08465) sdaAB 1622500..1623162 (+) 663 WP_019712516.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  PIB33_RS08470 (PIB33_08470) sdaAA 1623188..1624090 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  PIB33_RS08475 (PIB33_08475) recG 1624068..1626116 (+) 2049 WP_032721534.1 ATP-dependent DNA helicase RecG Machinery gene
  PIB33_RS08480 (PIB33_08480) fapR 1626225..1626791 (+) 567 WP_003232044.1 transcription factor FapR -
  PIB33_RS08485 (PIB33_08485) plsX 1626805..1627806 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  PIB33_RS08490 (PIB33_08490) fabD 1627825..1628778 (+) 954 WP_032721536.1 ACP S-malonyltransferase -
  PIB33_RS08495 (PIB33_08495) fabG 1628771..1629511 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  PIB33_RS08500 (PIB33_08500) acpP 1629595..1629828 (+) 234 WP_003154310.1 acyl carrier protein -
  PIB33_RS08505 (PIB33_08505) rncS 1629968..1630717 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78148.79 Da        Isoelectric Point: 7.4457

>NTDB_id=709724 PIB33_RS08475 WP_032721534.1 1624068..1626116(+) (recG) [Bacillus subtilis strain GXD-20]
MKQHQQTSIANIKGIGPETEKTLHELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKLGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=709724 PIB33_RS08475 WP_032721534.1 1624068..1626116(+) (recG) [Bacillus subtilis strain GXD-20]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTACACGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATTACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAGGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAACGT
TTTTCAAACGAAGAACTTATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAATCGTCTACTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGCCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCTAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAC
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CTACTGTTGTGGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGGCTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGAAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)

Helicase conserved C-terminal domain (Helicase_C)

(453-572)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.707

100

0.997
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38