Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   PIB33_RS06930 Genome accession   NZ_CP116391
Coordinates   1322115..1323464 (-) Length   449 a.a.
NCBI ID   WP_009967069.1    Uniprot ID   O34358
Organism   Bacillus subtilis strain GXD-20     
Function   degrading CSP; selective degradation of ComEA and ComEC (predicted from homology)   
Competence regulation

Genomic Context

Location: 1317115..1328464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS06905 (PIB33_06905) mhqA 1317259..1318209 (+) 951 WP_003245086.1 ring-cleaving dioxygenase -
  PIB33_RS06910 (PIB33_06910) - 1318310..1318456 (+) 147 WP_003244977.1 hypothetical protein -
  PIB33_RS06915 (PIB33_06915) ykcB 1318464..1320614 (+) 2151 WP_032721393.1 glycosyltransferase family 39 protein -
  PIB33_RS06920 (PIB33_06920) gtcC 1320626..1321597 (+) 972 WP_003232632.1 glycosyltransferase family 2 protein -
  PIB33_RS06925 (PIB33_06925) - 1321781..1321957 (-) 177 WP_003245413.1 hypothetical protein -
  PIB33_RS06930 (PIB33_06930) htrA 1322115..1323464 (-) 1350 WP_009967069.1 serine protease HtrA Regulator
  PIB33_RS06935 (PIB33_06935) proG 1323633..1324451 (+) 819 WP_032721395.1 pyrroline-5-carboxylate reductase ProG -
  PIB33_RS06940 (PIB33_06940) dppA 1324580..1325404 (+) 825 WP_032721396.1 D-aminopeptidase DppA -
  PIB33_RS06945 (PIB33_06945) dppB 1325421..1326347 (+) 927 WP_003245446.1 dipeptide ABC transporter permease DppB -
  PIB33_RS06950 (PIB33_06950) dppC 1326353..1327315 (+) 963 WP_032721397.1 dipeptide ABC transporter permease DppC -
  PIB33_RS06955 (PIB33_06955) dppD 1327320..1328327 (+) 1008 WP_032721399.1 dipeptide ABC transporter ATP-binding subunit DppD -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 47714.85 Da        Isoelectric Point: 4.5990

>NTDB_id=709710 PIB33_RS06930 WP_009967069.1 1322115..1323464(-) (htrA) [Bacillus subtilis strain GXD-20]
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=709710 PIB33_RS06930 WP_009967069.1 1322115..1323464(-) (htrA) [Bacillus subtilis strain GXD-20]
ATGGATAACTATCGTGATGAAAACAGAACGAAAGGTAATGAGAATGAGGTCTTTTTAACGAAAGAGAACGATCAGAGCGC
CTCCTACTCGGCCCGCAATGTCATTCATGATCAGGAGAAGAAAAAACGAGGATTCGGATGGTTCAGACCGTTGCTTGGCG
GAGTGATCGGCGGCAGTCTTGCTCTTGGCATTTACACGTTTACACCGCTTGGTGACCATGATTCTCAGGACACTGCAAAA
CAATCATCCAGCCAGCAGCAAACGCAATCTGTTACAGCAACAAGCACCTCCTCTGAATCTAAAAAAAGCTCAAGCAGCTC
ATCTGCATTCAAGAGCGAGGACTCTTCTAAAATCTCAGATATGGTAGAAGACCTTTCACCAGCGATTGTCGGTATTACAA
ATCTTCAGGCACAATCAAACAGCTCTTTGTTCGGCTCTAGTTCTTCTGATTCCAGCGAAGATACAGAAAGCGGTTCAGGG
TCAGGTGTCATTTTCAAAAAAGAGAATGGCAAGGCTTATATCATTACAAATAACCACGTCGTAGAAGGGGCATCATCACT
GAAGGTATCTTTATATGACGGCACTGAGGTTACTGCAAAGCTGGTAGGCAGTGACTCGTTAACTGATTTAGCCGTCCTCC
AAATCAGTGATGACCACGTCACAAAAGTGGCAAACTTCGGTGATTCATCTGATCTTAGAACAGGCGAGACCGTTATTGCG
ATTGGGGATCCGCTTGGAAAAGACCTGTCCCGCACAGTAACACAAGGAATTGTAAGCGGCGTGGACAGAACGGTTTCAAT
GTCTACATCAGCCGGCGAAACGAGCATTAACGTCATTCAGACAGACGCAGCAATTAATCCAGGTAACAGCGGCGGTCCTT
TGTTAAATACAGACGGCAAAATTGTCGGCATTAACAGTATGAAAATCAGTGAGGATGATGTTGAGGGTATCGGATTCGCC
ATTCCAAGCAATGACGTAAAACCGATTGCTGAAGAATTGCTGTCTAAAGGACAAATTGAACGTCCATATATCGGTGTCAG
CATGCTTGATCTAGAGCAAGTGCCGCAAAATTACCAAGAAGGCACACTCGGCCTGTTCGGCAGCCAGCTGAATAAAGGCG
TTTACATCCGTGAGGTCGCTTCAGGCTCTCCTGCTGAAAAGGCCGGATTAAAAGCGGAGGATATTATCATCGGCCTAAAA
GGTAAAGAAATTGATACAGGCAGTGAATTGCGCAATATCTTATATAAAGACGCAAAGATCGGTGATACCGTTGAAGTGAA
AATTCTCCGAAACGGCAAAGAAATGACGAAAAAAATTAAACTGGATCAAAAAGAAGAGAAAACTTCGTAA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(342-441)

Trypsin-like peptidase domain (Trypsin_2)

(160-304)

Peptidase family M50 (Peptidase_M50)

(366-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34358

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus pneumoniae Rx1

41.045

89.532

0.367
  htrA Streptococcus pneumoniae D39

41.045

89.532

0.367
  htrA Streptococcus pneumoniae R6

41.045

89.532

0.367
  htrA Streptococcus pneumoniae TIGR4

41.045

89.532

0.367
  htrA Streptococcus mitis NCTC 12261

39.563

91.759

0.363