Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NMT99_RS18220 Genome accession   NZ_CP101290
Coordinates   3513556..3514947 (-) Length   463 a.a.
NCBI ID   WP_029725993.1    Uniprot ID   -
Organism   Bacillus subtilis strain LjM2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3508556..3519947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS18185 flgL 3508730..3509626 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  NMT99_RS18190 flgK 3509637..3511160 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  NMT99_RS18195 flgN 3511179..3511661 (-) 483 WP_014481048.1 flagellar protein FlgN -
  NMT99_RS18200 flgM 3511677..3511943 (-) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  NMT99_RS18205 yvyF 3512024..3512440 (-) 417 WP_259597028.1 TIGR03826 family flagellar region protein -
  NMT99_RS18210 comFC 3512514..3513236 (-) 723 WP_029725992.1 comF operon protein ComFC Machinery gene
  NMT99_RS18215 comFB 3513200..3513496 (-) 297 WP_015483764.1 late competence protein ComFB -
  NMT99_RS18220 comFA 3513556..3514947 (-) 1392 WP_029725993.1 ATP-dependent helicase ComFA Machinery gene
  NMT99_RS18225 fakBA 3515053..3515898 (-) 846 WP_003227986.1 DegV family protein -
  NMT99_RS18230 degU 3515996..3516685 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  NMT99_RS18235 degS 3516768..3517925 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  NMT99_RS18240 yvyE 3518142..3518795 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52575.62 Da        Isoelectric Point: 9.9619

>NTDB_id=709504 NMT99_RS18220 WP_029725993.1 3513556..3514947(-) (comFA) [Bacillus subtilis strain LjM2]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNKLAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=709504 NMT99_RS18220 WP_029725993.1 3513556..3514947(-) (comFA) [Bacillus subtilis strain LjM2]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCCTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCGCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCATTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATA
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

99.136

100

0.991