NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	NMT99_RS12890	Genome accession	NZ_CP101290
Coordinates	2500348..2500965 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis strain LjM2
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2495348..2505965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMT99_RS12860	rpsT	2495657..2495923 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
NMT99_RS12865	holA	2495938..2496981 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
NMT99_RS12870	-	2497021..2497170 (-)	150	WP_003229985.1	hypothetical protein	-
NMT99_RS12875	yqzM	2497211..2497345 (+)	135	WP_003229983.1	YqzM family protein	-
NMT99_RS12880	comEC	2497385..2499715 (-)	2331	WP_080477815.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NMT99_RS12885	comEB	2499712..2500281 (-)	570	WP_015714288.1	ComE operon protein 2	-
NMT99_RS12890	comEA	2500348..2500965 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
NMT99_RS12895	comER	2501049..2501870 (+)	822	WP_004398597.1	late competence protein ComER	-
NMT99_RS12900	yqeM	2501936..2502679 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
NMT99_RS12905	rsfS	2502676..2503032 (-)	357	WP_003229971.1	ribosome silencing factor	-
NMT99_RS12910	yqeK	2503050..2503610 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NMT99_RS12915	nadD	2503600..2504169 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
NMT99_RS12920	yhbY	2504181..2504471 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
NMT99_RS12925	aroE	2504465..2505307 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=709488 NMT99_RS12890 WP_004398514.1 2500348..2500965(-) (comEA) [Bacillus subtilis strain LjM2]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=709488 NMT99_RS12890 WP_004398514.1 2500348..2500965(-) (comEA) [Bacillus subtilis strain LjM2]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0709488