Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K6J65_RS04385 Genome accession   NZ_AP018764
Coordinates   834798..835490 (+) Length   230 a.a.
NCBI ID   WP_105886719.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-1 isolate 13H34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 829798..840490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J65_RS04365 (CHBNII1_08440) ampD 831069..831629 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J65_RS04370 (CHBNII1_08450) pilA 831744..832193 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J65_RS04375 (CHBNII1_08460) pilB 832190..833584 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  K6J65_RS04380 (CHBNII1_08470) pilC 833581..834801 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  K6J65_RS04385 (CHBNII1_08480) pilD 834798..835490 (+) 693 WP_105886719.1 A24 family peptidase Machinery gene
  K6J65_RS04390 (CHBNII1_08490) rho 835544..836806 (-) 1263 WP_015701557.1 transcription termination factor Rho -
  K6J65_RS04395 (CHBNII1_08500) metJ 837054..837371 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J65_RS04400 (CHBNII1_08510) - 837385..837771 (-) 387 WP_105886720.1 Cu(I)-responsive transcriptional regulator -
  K6J65_RS04405 (CHBNII1_08520) - 837848..838054 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J65_RS04410 (CHBNII1_08530) - 838140..838346 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  K6J65_RS04415 (CHBNII1_08540) - 838432..838638 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  K6J65_RS04420 (CHBNII1_08550) - 838724..838930 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26743.73 Da        Isoelectric Point: 9.0977

>NTDB_id=70733 K6J65_RS04385 WP_105886719.1 834798..835490(+) (pilD) [Haemophilus influenzae strain CHBN-II-1 isolate 13H34]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYVELFPQNRSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWLAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAIIIYLVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=70733 K6J65_RS04385 WP_105886719.1 834798..835490(+) (pilD) [Haemophilus influenzae strain CHBN-II-1 isolate 13H34]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACACG
TTTACAGCAAGAGATTTATGCGACTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTACTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGCTTGCAAAATTCTATTATGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAGGAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCGGCTATCATTATTTATCTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

97.826

100

0.978
  pilD Haemophilus influenzae 86-028NP

96.957

100

0.97

Multiple sequence alignment