Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   NLY75_RS02270 Genome accession   NZ_CP100432
Coordinates   426931..428223 (+) Length   430 a.a.
NCBI ID   WP_023369491.1    Uniprot ID   A0A0Z8EEP0
Organism   Streptococcus suis T15     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421931..433223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLY75_RS02245 (NLY75_02245) - 422736..423377 (+) 642 WP_002937025.1 response regulator transcription factor -
  NLY75_RS02250 (NLY75_02250) - 423427..424827 (+) 1401 WP_023369485.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  NLY75_RS02255 (NLY75_02255) - 424829..425200 (+) 372 WP_002937017.1 S1 RNA-binding domain-containing protein -
  NLY75_RS02260 (NLY75_02260) cysK 425226..426152 (-) 927 WP_023369487.1 cysteine synthase A -
  NLY75_RS02265 (NLY75_02265) - 426242..426874 (-) 633 WP_023369489.1 YigZ family protein -
  NLY75_RS02270 (NLY75_02270) comFA/cflA 426931..428223 (+) 1293 WP_023369491.1 DEAD/DEAH box helicase Machinery gene
  NLY75_RS02275 (NLY75_02275) - 428216..428881 (+) 666 WP_023369493.1 ComF family protein -
  NLY75_RS02280 (NLY75_02280) raiA 428958..429500 (+) 543 WP_004194117.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48941.56 Da        Isoelectric Point: 9.0711

>NTDB_id=705307 NLY75_RS02270 WP_023369491.1 426931..428223(+) (comFA/cflA) [Streptococcus suis T15]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLIVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSCFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLFTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=705307 NLY75_RS02270 WP_023369491.1 426931..428223(+) (comFA/cflA) [Streptococcus suis T15]
ATGAAAGAATTAGAAAATTATTACGGTAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTGCGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAACAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCGAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACGGCAACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGCAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTATTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTTCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGTGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTTCACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTTGGCAGGAGTATAGAACGTCCGACTGGT
TTACTCCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(294-393)

Type III restriction enzyme, res subunit (ResIII)

(91-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z8EEP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.285

100

0.674
  comFA/cflA Streptococcus pneumoniae Rx1

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae D39

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae R6

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae TIGR4

66.821

100

0.67
  comFA/cflA Streptococcus mitis SK321

66.125

100

0.663
  comFA Lactococcus lactis subsp. cremoris KW2

53.904

92.326

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.106

100

0.384
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363