Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EL335_RS12740 Genome accession   NZ_AP018718
Coordinates   2532457..2533902 (+) Length   481 a.a.
NCBI ID   WP_126447483.1    Uniprot ID   -
Organism   Sulfuricystis multivorans strain J5B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2527457..2538902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL335_RS12705 nusB 2527830..2528297 (+) 468 WP_126447470.1 transcription antitermination factor NusB -
  EL335_RS12710 thiL 2528310..2529245 (+) 936 WP_126447471.1 thiamine-phosphate kinase -
  EL335_RS12715 - 2529252..2529755 (+) 504 WP_126447473.1 phosphatidylglycerophosphatase A -
  EL335_RS12720 - 2529757..2530251 (+) 495 WP_126447475.1 nicotinamide-nucleotide amidohydrolase family protein -
  EL335_RS12725 recA 2530280..2531299 (+) 1020 WP_126447477.1 recombinase RecA Machinery gene
  EL335_RS12730 recX 2531292..2531720 (+) 429 WP_284155511.1 recombination regulator RecX -
  EL335_RS12735 lptB 2531717..2532451 (+) 735 WP_126447481.1 LPS export ABC transporter ATP-binding protein -
  EL335_RS12740 rpoN/rpoN1 2532457..2533902 (+) 1446 WP_126447483.1 RNA polymerase factor sigma-54 Machinery gene
  EL335_RS12745 raiA 2533916..2534227 (+) 312 WP_126447485.1 ribosome-associated translation inhibitor RaiA -
  EL335_RS12750 ptsN 2534356..2534817 (+) 462 WP_126447487.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EL335_RS12755 hprK 2534805..2535743 (+) 939 WP_126447903.1 HPr(Ser) kinase/phosphatase -
  EL335_RS12760 - 2535815..2536237 (+) 423 WP_126447489.1 DUF971 domain-containing protein -
  EL335_RS12765 ubiE 2536250..2536981 (+) 732 WP_126447491.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  EL335_RS12770 - 2536981..2537550 (+) 570 WP_126447493.1 SCP2 sterol-binding domain-containing protein -
  EL335_RS12775 - 2537536..2538516 (-) 981 WP_126447495.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 53096.06 Da        Isoelectric Point: 5.0057

>NTDB_id=70464 EL335_RS12740 WP_126447483.1 2532457..2533902(+) (rpoN/rpoN1) [Sulfuricystis multivorans strain J5B]
MKQTLQLKLSQHLALTPQLQQSIRLLQLSTLELNQEIEQALQDNPLLEWAEPDDAHVFSGASDALSHANELAPSTGEEHI
ETTAAWSEDGLEWPSAGAKGPRDPNDENDADAGELAAAATSLTDHLIAQLALTQLAGRDRLLVAFLIEALDEDGYLGQSL
EELAEVLPPELIGEPDEVVDDLQIALKHLQSLDPTGVGARDARECLLLQLANLPATPERTLAMTIVRDHLEQLAAHDYAR
IKKATGCDDEALRNAQELIRSLNPRPGAQFAALDTRYVVPDVIVRKLRGQWVAQLNQDVMPRLRINRQYADLLRRHRASG
GNNLTGQLQEAKWLIKNVQQRFDTIARVAQAIVDRQRAFFDHGEVAMRPLTLREIAETVDLHESTISRVTTQKYLACPRG
VYELKYFFGSHVATDTGGAASSTAIRALIRQLVAAEDGQKPLSDARIADILGEQGFKVARRTVAKYRESLGIPPVNLRKT
L

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=70464 EL335_RS12740 WP_126447483.1 2532457..2533902(+) (rpoN/rpoN1) [Sulfuricystis multivorans strain J5B]
GTGAAGCAAACGCTCCAGCTCAAGCTGTCGCAGCATCTCGCACTGACGCCGCAGTTGCAGCAATCGATCCGCCTGTTGCA
ATTGTCGACGCTGGAGCTCAACCAGGAAATCGAGCAGGCGCTGCAGGACAATCCATTGCTCGAATGGGCAGAGCCGGATG
ATGCTCACGTCTTCAGCGGCGCTTCGGATGCCTTGAGCCATGCCAATGAACTAGCGCCATCCACTGGCGAGGAGCACATC
GAAACCACCGCCGCATGGAGCGAGGATGGCTTGGAGTGGCCCAGCGCTGGCGCCAAAGGGCCGCGTGATCCGAACGATGA
AAACGATGCCGATGCCGGCGAGCTCGCAGCCGCCGCGACGAGCCTCACCGATCACCTGATCGCCCAATTGGCCCTGACGC
AGCTTGCCGGGCGTGACCGGCTGCTCGTGGCGTTCCTGATCGAAGCGCTCGATGAGGACGGCTATCTCGGTCAGTCATTG
GAAGAACTCGCAGAAGTGCTGCCACCGGAACTGATCGGCGAACCAGATGAAGTCGTCGATGACTTGCAAATCGCGCTCAA
GCATTTACAAAGTCTCGATCCAACGGGCGTCGGAGCTCGCGATGCGCGCGAATGCCTGCTGCTCCAGCTCGCCAACTTAC
CGGCCACCCCGGAGCGCACGCTGGCCATGACGATCGTGCGCGACCACCTCGAACAATTGGCGGCACACGATTACGCGCGC
ATCAAAAAGGCGACGGGCTGTGACGACGAGGCATTGCGCAACGCCCAGGAGTTGATCCGTTCGCTCAACCCGCGGCCGGG
CGCGCAATTCGCGGCGCTCGACACGCGCTATGTCGTGCCCGACGTGATCGTCCGGAAACTGCGCGGCCAGTGGGTTGCCC
AGTTGAATCAGGATGTCATGCCGCGGCTGCGCATCAACCGTCAATACGCCGATCTGCTGCGCCGCCATCGCGCGAGCGGC
GGCAACAATCTCACCGGCCAGCTGCAGGAAGCCAAGTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACCATCGCCCG
CGTCGCACAAGCCATCGTCGACCGCCAGCGCGCCTTCTTCGACCACGGCGAGGTGGCGATGCGGCCATTGACACTGCGCG
AGATCGCCGAAACGGTCGATCTGCACGAATCGACCATCTCGCGCGTGACGACGCAGAAATACCTCGCCTGTCCGCGCGGT
GTCTATGAACTCAAATATTTTTTTGGCAGCCACGTCGCCACCGATACCGGCGGCGCGGCTTCATCCACCGCGATCCGGGC
GCTGATCAGGCAGCTCGTCGCCGCCGAAGATGGCCAAAAGCCGCTCTCCGACGCGCGCATCGCGGATATCCTCGGCGAAC
AGGGCTTCAAGGTCGCGCGGCGCACCGTCGCCAAGTACCGCGAATCTCTGGGTATTCCGCCGGTCAACCTTCGCAAAACT
CTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(325-479)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(114-309)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.708

100

0.607

Multiple sequence alignment