Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   NKZ66_RS02205 Genome accession   NZ_CP100336
Coordinates   427576..428868 (+) Length   430 a.a.
NCBI ID   WP_024415030.1    Uniprot ID   A0A0Z8MCD5
Organism   Streptococcus suis strain STC81     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422576..433868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKZ66_RS02180 (NKZ66_02180) - 423381..424022 (+) 642 WP_024394813.1 response regulator transcription factor -
  NKZ66_RS02185 (NKZ66_02185) - 424072..425472 (+) 1401 WP_024394814.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  NKZ66_RS02190 (NKZ66_02190) - 425474..425845 (+) 372 WP_024394815.1 S1 RNA-binding domain-containing protein -
  NKZ66_RS02195 (NKZ66_02195) cysK 425871..426797 (-) 927 WP_012774985.1 cysteine synthase A -
  NKZ66_RS02200 (NKZ66_02200) - 426887..427519 (-) 633 WP_015646510.1 YigZ family protein -
  NKZ66_RS02205 (NKZ66_02205) comFA/cflA 427576..428868 (+) 1293 WP_024415030.1 DEAD/DEAH box helicase Machinery gene
  NKZ66_RS02210 (NKZ66_02210) comFC/cflB 428861..429526 (+) 666 WP_004194119.1 ComF family protein Machinery gene
  NKZ66_RS02215 (NKZ66_02215) raiA 429603..430145 (+) 543 WP_004194117.1 ribosome-associated translation inhibitor RaiA -
  NKZ66_RS02220 (NKZ66_02220) - 430188..431141 (-) 954 WP_024376800.1 IS30 family transposase -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48897.45 Da        Isoelectric Point: 9.1612

>NTDB_id=704543 NKZ66_RS02205 WP_024415030.1 427576..428868(+) (comFA/cflA) [Streptococcus suis strain STC81]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=704543 NKZ66_RS02205 WP_024415030.1 427576..428868(+) (comFA/cflA) [Streptococcus suis strain STC81]
ATGAAAGAATTAGAAAATTATTACGGTAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTGCGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAACAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCGAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGCAAATTAAGCCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTTCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTCCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGAAGGAGTATAGAACGTCCGACTGGT
TTACTTCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-393)

Type III restriction enzyme, res subunit (ResIII)

(91-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z8MCD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

65.893

100

0.66
  comFA Lactococcus lactis subsp. cremoris KW2

54.156

92.326

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363