Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL335_RS07055 Genome accession   NZ_AP018718
Coordinates   1421312..1422403 (-) Length   363 a.a.
NCBI ID   WP_126445413.1    Uniprot ID   -
Organism   Sulfuricystis multivorans strain J5B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416312..1427403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL335_RS07025 thpR 1416828..1417388 (-) 561 WP_126445403.1 RNA 2',3'-cyclic phosphodiesterase -
  EL335_RS07030 ispF 1417381..1417863 (-) 483 WP_126445405.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EL335_RS07035 ispD 1417860..1418546 (-) 687 WP_126445407.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EL335_RS07040 amrB 1418639..1419457 (+) 819 WP_284155278.1 AmmeMemoRadiSam system protein B -
  EL335_RS07045 amrA 1419450..1420007 (+) 558 WP_126445409.1 AmmeMemoRadiSam system protein A -
  EL335_RS07050 amrS 1419983..1421077 (+) 1095 WP_126445411.1 AmmeMemoRadiSam system radical SAM enzyme -
  EL335_RS07055 pilU 1421312..1422403 (-) 1092 WP_126445413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL335_RS07060 - 1422495..1423244 (+) 750 WP_126445415.1 fused MFS/spermidine synthase -
  EL335_RS07065 - 1423265..1423612 (+) 348 WP_126445417.1 ArsC family reductase -
  EL335_RS07070 - 1423612..1424448 (+) 837 WP_126445419.1 Hsp33 family molecular chaperone HslO -
  EL335_RS07075 gcvT 1424786..1425883 (+) 1098 WP_126445421.1 glycine cleavage system aminomethyltransferase GcvT -
  EL335_RS07080 gcvH 1425880..1426260 (+) 381 WP_126445423.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40028.17 Da        Isoelectric Point: 6.8286

>NTDB_id=70453 EL335_RS07055 WP_126445413.1 1421312..1422403(-) (pilU) [Sulfuricystis multivorans strain J5B]
MTEIQELLHQMLERGASDLFISAEIPPHLKIQGRTLPAQRAGGSWPALAPGESRRFAYALMNEAQRAEFEKNKECNFALA
TREGGRYRVNVYYQRGEVSMVIRLIKTDVPSFEVLGLPPQAGQLALLRRGLVLIVGAAGMGKSTTLAAMVAHRARTLDGH
ILSVEDPIEYIYPHGKSVVEQREVGIDTLSFADALKNMMRQAPDVIVIGEIRDRETAKNAIMYAEAGALCLSTLHSNNAN
QAIERIVNFFPEELHHQVLLDLSLNLRGVISQRLLPGLHGGQVLATEILLQSAYSSSLIQKGEIDQLKDAIRKGTASGMQ
LFDDCLLELCRARRIAPEEALDHADSKTDLALKLRLAVAPERL

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=70453 EL335_RS07055 WP_126445413.1 1421312..1422403(-) (pilU) [Sulfuricystis multivorans strain J5B]
ATGACCGAAATCCAGGAGCTGTTGCACCAAATGCTCGAGCGCGGGGCATCCGACCTGTTCATCTCTGCGGAGATTCCACC
CCATTTGAAGATTCAAGGCCGAACACTCCCGGCGCAGCGGGCAGGTGGATCATGGCCTGCCCTGGCGCCTGGCGAATCAC
GTCGCTTCGCGTATGCCTTGATGAATGAAGCGCAGCGCGCCGAATTCGAAAAGAACAAGGAATGCAATTTCGCACTCGCC
ACGCGCGAGGGTGGACGTTATCGCGTCAACGTCTATTACCAGCGCGGTGAGGTGTCGATGGTGATCCGTCTGATCAAGAC
GGACGTCCCATCATTCGAGGTGCTGGGCTTGCCCCCCCAGGCGGGGCAGCTTGCATTGTTGCGGCGCGGGCTGGTGCTGA
TCGTCGGCGCGGCGGGGATGGGCAAGAGCACCACGCTGGCGGCGATGGTCGCGCACCGTGCGCGGACGCTGGACGGCCAC
ATCCTTTCGGTCGAAGACCCGATCGAGTACATCTATCCACACGGCAAGTCGGTCGTCGAGCAGCGCGAAGTGGGGATCGA
TACCTTGAGCTTTGCCGATGCGCTCAAGAACATGATGCGCCAGGCCCCGGACGTGATCGTCATCGGCGAGATCCGCGACC
GCGAGACAGCCAAAAACGCCATCATGTATGCCGAAGCCGGTGCCTTGTGTCTGTCGACGCTGCACTCGAACAATGCCAAT
CAGGCGATCGAACGCATCGTCAATTTCTTTCCCGAGGAATTGCATCATCAGGTGTTGCTCGATCTGTCGCTCAACCTCAG
GGGTGTCATCTCGCAAAGACTCCTGCCAGGACTGCACGGTGGCCAGGTGTTGGCGACCGAAATCTTGCTGCAGTCGGCCT
ATTCGTCGTCGCTGATCCAAAAAGGCGAGATCGATCAGCTCAAGGACGCGATCCGCAAGGGAACCGCCAGCGGCATGCAA
TTGTTCGACGACTGCCTGCTGGAACTATGCCGCGCCCGGCGCATCGCGCCCGAAGAGGCGCTCGATCATGCCGATTCGAA
GACCGATCTCGCGCTCAAGCTGCGGCTGGCCGTCGCTCCCGAACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

49.438

98.072

0.485
  pilU Vibrio cholerae strain A1552

46.893

97.521

0.457
  pilU Acinetobacter baylyi ADP1

44.693

98.623

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

96.419

0.41
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.746

96.694

0.375
  pilT Vibrio cholerae strain A1552

38.746

96.694

0.375
  pilT Legionella pneumophila strain ERS1305867

39.017

95.317

0.372
  pilT Legionella pneumophila strain Lp02

39.017

95.317

0.372
  pilT Pseudomonas aeruginosa PAK

38.596

94.215

0.364
  pilT Neisseria meningitidis 8013

37.322

96.694

0.361
  pilT Neisseria gonorrhoeae MS11

37.11

97.245

0.361

Multiple sequence alignment