Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   PCS81_RS09985 Genome accession   NZ_CP115342
Coordinates   2082202..2084034 (+) Length   610 a.a.
NCBI ID   WP_001283421.1    Uniprot ID   P0A8G1
Organism   Escherichia coli strain CUVET21-1780     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2077202..2089034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS81_RS09950 (PCS81_09945) dcyD 2077429..2078415 (+) 987 WP_001128235.1 D-cysteine desulfhydrase -
  PCS81_RS09955 (PCS81_09950) tcyL 2078430..2079098 (+) 669 WP_001158220.1 cystine ABC transporter permease -
  PCS81_RS09960 (PCS81_09955) tcyN 2079095..2079847 (+) 753 WP_001272994.1 L-cystine ABC transporter ATP-binding protein TcyN -
  PCS81_RS09965 (PCS81_09960) sdiA 2080077..2080799 (+) 723 WP_001154255.1 transcriptional regulator SdiA -
  PCS81_RS09970 (PCS81_09965) yecF 2080866..2081090 (-) 225 WP_000106474.1 DUF2594 family protein YecF -
  PCS81_RS09975 (PCS81_09970) yecU 2081077..2081253 (-) 177 WP_001307856.1 protein YecU -
  PCS81_RS09980 (PCS81_09975) letA 2081549..2082205 (+) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  PCS81_RS09985 (PCS81_09980) uvrC 2082202..2084034 (+) 1833 WP_001283421.1 excinuclease ABC subunit UvrC Machinery gene
  PCS81_RS09990 (PCS81_09985) pgsA 2084091..2084639 (+) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  PCS81_RS10010 (PCS81_10005) - 2085337..2085978 (-) 642 Protein_1950 hypothetical protein -
  PCS81_RS10015 (PCS81_10010) - 2086051..2087139 (+) 1089 Protein_1951 IS3 family transposase -
  PCS81_RS10020 (PCS81_10015) - 2087491..2089014 (+) 1524 WP_000998248.1 type I restriction-modification system subunit M -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68188.03 Da        Isoelectric Point: 9.3685

>NTDB_id=703446 PCS81_RS09985 WP_001283421.1 2082202..2084034(+) (uvrC) [Escherichia coli strain CUVET21-1780]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=703446 PCS81_RS09985 WP_001283421.1 2082202..2084034(+) (uvrC) [Escherichia coli strain CUVET21-1780]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCTTATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCGAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGTTACTGCAAAAGATATTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGC
AATCGCTCGCGTCCGTGTCTGCAATATCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGCCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCGGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAGTTTGTGTCCAATACCGGCGACGACCTTGACGTTATTGGTGTGGCGTTTGATGCGGGCATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGTAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTGGAAACCTTCGTAGGTCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGCGGCGATAGGGCGCGTTATCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGACTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGTGAACAAACCGTCGCCTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACTGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TTGACGACAGTAAGATCCCGGATGTGATCCTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCGCTGCTACTTGGCGTTGCCAAAGGAGCAGACCGTAAAGCTGGGCTGGA
AACGCTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCTCACGATCACGCGATTGGTGGGCACCGTAAGAAACGGGCGAAGGTCAAAAATACCAGTTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGTCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
TGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A8G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374