Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NG812_RS04185 Genome accession   NZ_CP099987
Coordinates   834398..835654 (-) Length   418 a.a.
NCBI ID   WP_165705905.1    Uniprot ID   A0AAX3NCX3
Organism   Lactococcus garvieae strain FUA009     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 829398..840654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG812_RS04150 (NG812_04135) rpmA 829519..829803 (+) 285 WP_004257956.1 50S ribosomal protein L27 -
  NG812_RS04155 (NG812_04140) ald 830153..831259 (+) 1107 WP_165705902.1 alanine dehydrogenase -
  NG812_RS04160 (NG812_04145) - 831377..832336 (+) 960 WP_393187404.1 PhoH family protein -
  NG812_RS04165 (NG812_04150) - 832338..832811 (+) 474 WP_014024618.1 NUDIX hydrolase -
  NG812_RS04170 (NG812_04155) ybeY 832821..833309 (+) 489 WP_165705903.1 rRNA maturation RNase YbeY -
  NG812_RS04175 (NG812_04160) - 833293..833754 (+) 462 WP_042218685.1 diacylglycerol kinase family protein -
  NG812_RS04180 (NG812_04165) - 833757..834401 (-) 645 WP_165705904.1 ComF family protein -
  NG812_RS04185 (NG812_04170) comFA 834398..835654 (-) 1257 WP_165705905.1 DEAD/DEAH box helicase Machinery gene
  NG812_RS04190 (NG812_04175) - 835709..836332 (+) 624 WP_004257938.1 YigZ family protein -
  NG812_RS04195 (NG812_04180) - 836346..837368 (+) 1023 WP_165705906.1 DUF475 domain-containing protein -
  NG812_RS04200 (NG812_04185) - 837399..838421 (-) 1023 WP_270252753.1 alpha/beta hydrolase -
  NG812_RS04205 (NG812_04190) - 838741..839520 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  NG812_RS04210 (NG812_04195) glyQ 839534..840502 (+) 969 WP_004257927.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47801.46 Da        Isoelectric Point: 9.8475

>NTDB_id=703199 NG812_RS04185 WP_165705905.1 834398..835654(-) (comFA) [Lactococcus garvieae strain FUA009]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASSLNWQGKLTASQQHVSDSLVKLHQQQKTVLVQAVTGAGKTEMIYQSIDNTLKEGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKIVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSPERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=703199 NG812_RS04185 WP_165705905.1 834398..835654(-) (comFA) [Lactococcus garvieae strain FUA009]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATCAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTGCGCTCGGATGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTCTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAAATT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCTGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACCCTTAAAGAAGGAAAGGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCGCCACTCGTAATTGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGTTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATAGTCAAAAAACAACGCGAATCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACAAGTCCTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATCTAGGAAAAACAACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGGGGGTTCCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(92-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.653

93.78

0.541
  comFA/cflA Streptococcus mitis SK321

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae TIGR4

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae Rx1

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae D39

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae R6

52.347

100

0.533
  comFA/cflA Streptococcus mitis NCTC 12261

51.408

100

0.524
  comFA Bacillus subtilis subsp. subtilis str. 168

38.861

96.651

0.376
  comFA Latilactobacillus sakei subsp. sakei 23K

38.539

94.976

0.366