Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI383_RS21735 Genome accession   NZ_CP099955
Coordinates   2926124..2926717 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain Isc4A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2921124..2931717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI383_RS21715 (NI383_21870) aroB 2922000..2923100 (-) 1101 WP_025504649.1 3-dehydroquinate synthase -
  NI383_RS21720 (NI383_21875) aroK 2923132..2923650 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI383_RS21725 (NI383_21880) pilQ 2923839..2925587 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI383_RS21730 (NI383_21885) pilP 2925619..2926134 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  NI383_RS21735 (NI383_21890) pilO 2926124..2926717 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI383_RS21740 (NI383_21895) pilN 2926710..2927288 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  NI383_RS21745 (NI383_21900) pilM 2927272..2928288 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene
  NI383_RS21750 (NI383_21905) - 2928431..2931001 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=702906 NI383_RS21735 WP_005480482.1 2926124..2926717(-) (pilO) [Vibrio parahaemolyticus strain Isc4A]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=702906 NI383_RS21735 WP_005480482.1 2926124..2926717(-) (pilO) [Vibrio parahaemolyticus strain Isc4A]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTATATCGATTGC
CGCTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726