Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NI383_RS21745 Genome accession   NZ_CP099955
Coordinates   2927272..2928288 (-) Length   338 a.a.
NCBI ID   WP_005480475.1    Uniprot ID   A0A0L7V0I6
Organism   Vibrio parahaemolyticus strain Isc4A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2922272..2933288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI383_RS21720 (NI383_21875) aroK 2923132..2923650 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI383_RS21725 (NI383_21880) pilQ 2923839..2925587 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI383_RS21730 (NI383_21885) pilP 2925619..2926134 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  NI383_RS21735 (NI383_21890) pilO 2926124..2926717 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI383_RS21740 (NI383_21895) pilN 2926710..2927288 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  NI383_RS21745 (NI383_21900) pilM 2927272..2928288 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene
  NI383_RS21750 (NI383_21905) - 2928431..2931001 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NI383_RS21755 (NI383_21910) oxyR 2931052..2931960 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  NI383_RS21760 (NI383_21915) - 2932131..2932859 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.31 Da        Isoelectric Point: 6.8323

>NTDB_id=702908 NI383_RS21745 WP_005480475.1 2927272..2928288(-) (pilM) [Vibrio parahaemolyticus strain Isc4A]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=702908 NI383_RS21745 WP_005480475.1 2927272..2928288(-) (pilM) [Vibrio parahaemolyticus strain Isc4A]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L7V0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482