Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI380_RS01595 Genome accession   NZ_CP099934
Coordinates   336859..337452 (+) Length   197 a.a.
NCBI ID   WP_005496571.1    Uniprot ID   A0A0F2HLP7
Organism   Vibrio parahaemolyticus strain BM26A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331859..342452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI380_RS01580 (NI380_01580) - 332575..335145 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NI380_RS01585 (NI380_01585) pilM 335288..336304 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NI380_RS01590 (NI380_01590) pilN 336288..336866 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NI380_RS01595 (NI380_01595) pilO 336859..337452 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI380_RS01600 (NI380_01600) pilP 337442..337957 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  NI380_RS01605 (NI380_01605) pilQ 337989..339737 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI380_RS01610 (NI380_01610) aroK 339926..340444 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NI380_RS01615 (NI380_01615) aroB 340476..341576 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23019.57 Da        Isoelectric Point: 4.9419

>NTDB_id=702711 NI380_RS01595 WP_005496571.1 336859..337452(+) (pilO) [Vibrio parahaemolyticus strain BM26A]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDGK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=702711 NI380_RS01595 WP_005496571.1 336859..337452(+) (pilO) [Vibrio parahaemolyticus strain BM26A]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTTACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGCATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGGGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2HLP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711