Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   NI384_RS01595 Genome accession   NZ_CP099928
Coordinates   336172..336750 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain BM24B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331172..341750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI384_RS01580 (NI384_01580) oxyR 331495..332403 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  NI384_RS01585 (NI384_01585) - 332459..335029 (-) 2571 WP_308385385.1 PBP1A family penicillin-binding protein -
  NI384_RS01590 (NI384_01590) pilM 335172..336188 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NI384_RS01595 (NI384_01595) pilN 336172..336750 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NI384_RS01600 (NI384_01600) pilO 336743..337336 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI384_RS01605 (NI384_01605) pilP 337326..337841 (+) 516 WP_025794802.1 pilus assembly protein PilP Machinery gene
  NI384_RS01610 (NI384_01610) pilQ 337873..339621 (+) 1749 WP_308385386.1 type IV pilus secretin PilQ family protein Machinery gene
  NI384_RS01615 (NI384_01615) aroK 339810..340328 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NI384_RS01620 (NI384_01620) aroB 340360..341460 (+) 1101 WP_140244873.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=702665 NI384_RS01595 WP_015297396.1 336172..336750(+) (pilN) [Vibrio parahaemolyticus strain BM24B]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=702665 NI384_RS01595 WP_015297396.1 336172..336750(+) (pilN) [Vibrio parahaemolyticus strain BM24B]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTCAACACAGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594